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Publikationen der AG Wahle

2023

Pekovic, F., Rammelt, C., Kubíková, J., Metz, J., Jeske, M., Wahle E. (2023)RNA binding proteins Smaug and Cup induce CCR4-NOT-dependent deadenylation of the nanos mRNA in a reconstituted system. Nucleic Acids Res. Online ahead of print

2022

Schmidt, M., Kluge, F., Sandmeir, F., Kühn, U.,  Schäfer, P., Tüting, C., Ihling, C.,  Conti, E., Wahle, E. (2022)  Reconstitution of 3' end processing of mammalian  pre-mRNA reveals a central role of RBBP6. Genes Dev. 36, 195-209.  doi:10.1101/gad.349276.121

2020

Kluge, F., Götze, M., Wahle, E. (2020)  Establishment of 5’-3’ interactions in mRNA independent of a continuous  ribose-phosphate backbone. RNA 26,  613-628.

Cao, W. X., Kabelitz, S. Gupta, M.,  Yeung, E., Lin, S., Rammelt, C ., Ihling, C., Pekovic, F., Low, T. C. H., Siddiqui, N.  U., Cheng, M. H. K., Anger, S., Smibert, C. A., Wühr, M., Wahle, E.*, Lipshitz,  H. D.* (2020) Precise temporal regulation of post-transcriptional repressors is  required for an orderly Drosophila maternal-to-zygotic transition. Cell Rep. 31,  107783.      *corresponding authors

2018

Schäfer, P., Tüting, C., Schönemann, L., Kühn, U., Treiber, T., Treiber, N., Ihling, C., Graber, A., Keller, W., Meister, G., Sinz, A., Wahle, E. (2018) Reconstitution of mammalian cleavage factor II involved in 3' processing of mRNA precursors. RNA 24, 1721-1737.

2017

Kühn, U., Buschmann, J., Wahle, E. (2017) The nuclear poly(A) binding protein of mammals, but not of fission yeast, participates in mRNA polyadenylation. RNA 23,  473-482.

Gehring, N. H., Wahle, E., Fischer, U.  (2017) Deciphering the mRNP code: RNA-bound determinants of post-transcriptional  gene regulation. Trends Biochem. Sci. 42,  369-382.

Götze, M., Dufourt, J., Ihling,  C., Rammelt, C.,  Pierson, S., Sambrani, N., Temme, C., Sinz, A., Simonelig, M., Wahle, E. (2017)  Translational repression of the Drosophila nanos mRNA  involves the RNA helicase Belle and RNA coating by Me31B and Trailer hitch. RNA 23, 1552-1568.

2016

Harnisch, C., Cuzic-Feltens, S., Dohm,  J. C., Götze, M., Himmelbauer, H., Wahle, E. (2016) Oligoadenylation of 3' decay  intermediates promotes cytoplasmic mRNA degradation in Drosophila cells. RNA 22,  428-442.

2015

Götze, M., Pettelkau, J., Fritzsche, R., Ihling, C.H., Schäfer, M., Sinz, A.(2015) Automated Assignment of MS/MS Cleavable Cross-Links in Protein 3D-Structure Analysis. J Am Soc Mass Spectrom. 26, 83-97.

Fiedler, M.,  Rossmanith, W., Wahle, E., Rammelt, C. (2015) Mitochondrial poly(A) polymerase  is involved in tRNA repair. Nucleic Acids Res. 43,  9937-9949.

2014

Moritz, B., Wahle, E. (2014) Simple methods for the 3' biotinylation of RNA. RNA 20, 421-427.

Götze, M., Wahle, E. (2014) Smaug destroys a huge treasure. Genome biology 15: 101.

Temme, C., Wahle, E. (2014) Assaying mRNA deadenylation in vivo. Methods Mol Biol. 1125, 313-324.

Jeske, M., Temme, C., Wahle, E. (2014) Assaying mRNA deadenylation in vitro. Methods Mol Biol. 1125, 297-311.

Monecke,  T., Buschmann, J., Neumann, P., Wahle, E., Ficner, R. (2014) Crystal  structures of the novel cytosolic 5'-nucleotidase IIIB explain its  preference for m7GMP. PLoS One. 9:e90915. doi: 10.1371

Temme, C., Simonelig, M., Wahle, E. (2014) Deadenylation of mRNA by the CCR4-NOT complexin Drosophila: molecular and developmental aspects. Front Genet.  5, 143.

Stubbs, M.T., Wahle, E. (2014) Deadenylation-a piece of PANcake. EMBO J. 33, 1503-1505.

Moritz, B., Lilie, H., Naarmann-de Vries, I.S., Urlaub, H., Wahle, E., Ostareck-Lederer, A., Ostareck, D.H.  (2014) Biophysical and biochemical analysis of hnRNP K: arginine  methylation, reversible aggregation and combinatorial binding to nucleic  acids. Biol Chem. 395, 837-853.

Schönemann, L., Kühn, U., Martin, G., Schäfer, P., Gruber A. R., Keller,
W., Zavolan, M., Wahle, E. (2014) Reconstitution of CPSF active in
polyadenylation: Recognition of the polyadenylation signal by WDR33.
Genes & Development 28, 2381-2393

2013

Harnisch,  C., Moritz, B., Rammelt, C., Temme, C., Wahle, E. (2013) Activity and function  of deadenylases. In: G.  Chanfreau, F.  Tamanoi, eds.: The Enzymes, Vol. 31, Eukaryotic RNases  and their partners in RNA  degradation and biogenesis, Part A.  Burlington: Academic Press, pp.  181-211.

Buschmann,  J., Moritz, B., Jeske, M., Lilie, H., Schierhorn, A., Wahle, E. (2013)  Identification of Drosophila and human 7-methyl GMP-specific nucleotidases. J.  Biol. Chem. 288, 2441-2451

Wahle E, Winkler GS. (2013) RNA decay machines: deadenylation by the Ccr4-not and Pan2-Pan3 complexes.Biochim Biophys Acta. 1829, 561-570.

2012

Berndt, H.,  Harnisch, C., Rammelt, C., Stöhr, N.,   Zirkel, A., Dohm, J. C., Himmelbauer, H.,  Tavanez, J.-P., Hüttelmaier,   S., Wahle, E. (2012) Maturation of mammalian H/ACA  box snoRNAs:  PAPD5-dependent adenylation and PARN-dependent trimming. RNA, 18,  958-972.

Jenal, M., Elkon, R., Loayza-Puch, F., van Haaften, G., Kühn, U.,  Menzies, F.M., Vrielink, J.A., Bos, A.J., Drost, J., Rooijers, K.,  Rubinsztein, D.C.,  Agami, R. (2012) The poly(a)-binding protein nuclear  1 suppresses  alternative cleavage and polyadenylation sites. Cell 149,  538-53.

Winter, R., Kühn, U., Hause, G., Schwarz, E. (2012)  Poly-Alanine-independent conformational conversion of the nuclear  poly(A) binding protein 1 (PABPN1).  J. Biol. Chem. 287: 22662-71.

Kölbel, K., Ihling, C., Kühn, U., Neundorf, I., Otto, S., Stichel,  J., Robaa, D., Beck-Sickinger, A.G.,  Sinz, A., Wahle, E. (2012) Peptide  backbone conformation affects the  substrate preference of protein  arginine methyltransferase I.  Biochemistry 51: 5463-75.

2011

Jeske, M., Moritz B., Anders, A., Wahle, E. (2011) Smaug assembles an ATP-dependent stable complex repressing nanos mRNA translation at multiple levels. EMBO J. 30, 90–103.

Fronz,  K., Güttinger, S., Burkert, K., Kühn, U., Stöhr, N., Schierhorn,  A.,  Wahle, E (2011) Arginine methylation of the nuclear poly(a) binding   protein weakens the interaction with its nuclear import receptor,   transportin. J. Biol. Chem. 286, 32986-32994.

Eckmann, C. R., Rammelt, C., Wahle,  E. (2011) Control of poly(A) tail length. WIREs RNA 2011, 2: 348-361.

Götze, M., Pettelkau, J., Schaks, S., Bosse, K., Ihling, C.H.,Krauth,   F., Fritzsche, R., Kühn, U., Sinz, A. (2011) StavroX—A Software for   Analyzing Crosslinked Products in Protein Interaction Studies. J. Am.   Soc. Mass Spectrom. 23, 76-87.

2010

Temme, C., Zhang, L., Kremmer, E., Ihling, C., Chartier, A., Sinz, A.,  Simonelig, M., Wahle, E. (2010) Subunits of the Drosophila CCR4-NOT complex and their roles in mRNA deadenylation. RNA 16, 1356-1370

2009

Hägele, S., Kühn, U., Böning, M., Katschinski, D. M.  (2009)  Cytoplasmic polyadenylation element binding protein (CPEB)1 and 2 bind  to the HIF-1α mRNA 3`UTR and modulate HIF-1α protein expression. Biochemical Journal 417, 235-246.

Weidensdorfer,  D., Stöhr, N., Baude, A., Lederer, M., Köhn, M., Schierhorn, A.,  Buchmeier, S., Wahle, E., Hüttelmaier, S. (2009) Control of c-myc mRNA stability by IGF2BP1 associated cytoplasmic RNPs. RNA 15, 104-115.

Temme,  C., Weissbach, R., Lilie, H., Wilson, C.,   Meinhart, A., Meyer,  S.,  Golbik, R., Schierhorn, A.,   Wahle, E. (2009) The Drosophila  melanogaster gene   CG4930 encodes a high affinity inhibitor for  endonuclease G. J. Biol. Chem., 284, 8337-8348.

Kölbel, K., Ihling, C., Bellmann-Sickert, K., Neundorf, I., Beck-Sickinger,  A. G., Sinz, A., Kühn, U., Wahle, E. (2009) Type I arginine methyl  transferases PRMT1 and 3 act distributively. J. Biol. Chem., 284, 8274-8282

Kühn,  U., Gündel, M. Knoth, A. Kerwitz; Y. Rüdel, S. Wahle, E. (2009)   Poly(A) tail length is controlled by the nuclear poly(A) binding   protein regulating the interaction between poly(A) polymerase and the   cleavage and polyadenylation specificity factor. J. Biol. Chem., 284, 22803-22814.

Loll,   B., Gebhardt, M., Wahle, E., Meinhart, A. (2009) Crystal structure of   the EndoG/EndoGI complex: Mechanism of EndoG inhibition. Nucleic Acids   Res., 3/, 7312-7320.

2008

Lodderstedt, G., Sachs, R., Faust, J., Bordusa, F., Kühn, U., Golbik, R., Kerth, A., Wahle, E., Balbach, J., Schwarz, E. (2008) Hofmeister Salts and Potential Therapeutic Compounds Accelerate in Vitro Fibril Formation of the N-Terminal Domain of PABPN1 Containing a Disease-Causing Alanine Extension. Biochemistry 47, 2181-2189.

Fronz, K., Otto,S.,  Kölbel, K., Kühn, U., Friedrich,  H., Schierhorn, A., Beck-Sickinger, A.-G., Ostareck-Lederer, A., Wahle, E. (2008) Promiscuous modification of the nuclear poly(A) binding protein by multiple protein arginine methyl transferases does not affect the aggregation behavior. J. Biol. Chem. 283, 20408-20420

Jeske, M., Wahle, E. (2008) Cell-free deadenylation assays using Drosophila embryo extracts. Methods Enzymol. 448, 107-118.
Meyer, S., Urbanke, C., Wahle, E. (2002) Equilibrium studies on the association of the nuclear poly(A) binding protein with poly(A) of different lengths. Biochem. 41, 6082-6089.

2007

Wahle, E. (2007) Wrong PH for RNA degradation. Nature Structural & Molecular Biology 14, 5-7.

Adolph, D., Flach, N., Mueller, K., Ostareck, D. H.,  Ostareck-Lederer, A.  (2007) Deciphering the cross talk between hnRNP K and c-Src: the c-Src activation domain in hnRNP K is destinct from a second interaction site. Molecular and Cellular Biology 27, 1758-1770.

Bönisch,  C., Temme, C., Moritz, B, Wahle, E. (2007) Degradation of hsp70 and  other mRNAs in Drosophila via the 5`-3` pathway and its regulation by  heat shock. J. Biol. Chem. 283, 21818-21828

2006

Ostareck-Lederer, A., Ostareck, D.H., Rücknagel, K.P.,  Schierhorn, A., Moritz, B., Hüttelmaier, S., Flach, N., Handoko, L. and  Wahle, E. (2006) Asymmetric arginine dimethylation of hnRNP K by PRMT1  inhibits its interaction with c-Src.  J. Biol. Chem. 281, 11115-11125.

Jeske, M., Meyer, S., Temme, C., Freudenreich, D., Wahle, E. (2006) Rapid ATP-dependent deadenylation of nanos mRNA in a cell-free system from Drosophila embryos. J. Biol. Chem. 281, 25124-25133.

Wahle,  E., Gehring, N. H. (2006) Polyadenylation. In: Ruckpaul, K. und Ganten,  D. (Herausg.), Encyclopedic Reference of Genomics and Proteomics in  Molecular Medicine. Springer Verlag, Heidelberg.

Stöhr, N.,  Lederer, M., Reinke, C., Meyer, S., Hatzfeld, M., Singer, R. H.,  Hüttelmaier, S. (2006) ZBP1 regulates mRNA stability during cellular  stress. J. Cell Biol., 175, 527-534.

Messias, A., Harnisch, C., Ostareck-Lederer, A., Sattler, M. and Ostareck, D.H. (2006) The DICE-binding activity of KH domain 3 of hnRNP K is affected by c-Src mediated tyrosine phosphorylation. J. Mol. Biol. 361, 470-481

2005

Benoit B, Mitou G, Chartier A, Temme C, Zaessinger S, Wahle E, Busseau I, Simonelig M. (2005) An essential cytoplasmic function for the nuclear poly(A) binding protein, PABP2, in poly(A) tail length control and early development in Drosophila. Dev Cell. 9, 511-22.

Wu M, Reuter M, Lilie H, Liu Y, Wahle E, Song H. (2005) Structural insight into poly(A) binding and catalytic mechanism of human PARN. EMBO J. 24, 4082-93.

Boehringer, D., Thermann, R., Ostareck-Lederer, A., Lewis, J.D. and Stark, H. (2005) Structure of the Hepatitis C virus IRES bound to the human 80S ribosome: Remodelling of the HCV IHRES.  Structure 13, 1695-1706

2004

Kühn, U., Wahle, E. (2004) Structure and function of poly(A) binding proteins. Biochim. Biophys. Acta 1678, 67-84.

Temme,  C., Zaessinger, S., Meyer, S., Simonelig, M., Wahle, E. (2004) A  complex containing the CCR4 and CAF1 proteins is involved in mRNA  deadenylation in Drosophila. EMBO J. 24, 4082-93.

Ostareck-Lederer, A. and Ostareck, D.H. (204) Control of mRNA translation and stability in haematopoietic cells: Functions of hnRNPs K and E1/2. Biol. Cell 96, 407-411 .

Ostareck-Lederer, A., Clauder-Münster, S., Thermann, R., Polycarpou-Schwarz, M., Gentzel, M., Wilm, M. and Lewis, J.D.  (2004) The role of RNA interference in drug target validation:  application to Hepatitis C. In: RNA Interference: From Basic Science to  Drug Development. (ed. K. Appasani), Cambridge University Press, 318-330.

Ostareck-Lederer, A. and Ostareck, D.H. (2004) Cellular signals controlling post-transcriptional regulation of reticulocyte-15-lipoxygenase (r15-LOX) expression during erythroid cell maturation.  Nova Acta Leopoldina 18, 163-178.

Thoma, C., Ostareck-Lederer, A. and Hentze, M.W.  (2004) A poly(A) tail responsive in vitro translation system in HeLa  cells. In: mRNA Processing and Metabolism – Methods and Protocols. (ed.  D.R. Schoenberg), The Human Press Inc. New York 2004, 171-180

2003

Kühn, U., Nemeth, A., Meyer, S., Wahle, E. (2003) The RNA  binding domains of the nuclear poly(A) binding protein. J. Biol. Chem.  278, 16916-16925.

Kerwitz, Y., Kühn, U., Lilie, H., Knoth, A.,  Scheuermann, T., Friedrich, H., Schwarz, E., Wahle, E. (2003)  Stimulation of poly(A) polymerase through a direct interaction with the  nuclear poly(A) binding protein allosterically regulated by poly(A).  EMBO J. 22, 3705-3714.

Scheuermann, T., Schulz, B., Blume,  A., Wahle, E., Rudolph, R., Schwarz, E. (2003) Trinucleotide expansions  leading to an extended poly-L-alanine segment in the poly(A) binding protein PABPN1 cause fibril formation. Protein Science 12, 2685-2692.

Evans J.R., Mitchell, S.A., Spriggs, K.A., Ostrowsky, J., Bomsztyk, K., Ostareck, D.H., and Willis, A.E. (2003) Members of the poly rC binding protein family stimulate the activity of the c-myc internal ribosome entry segment in vitro and in vivo. Oncogene 22, 8012-8020

2002

Calapez, A., Pereira, H. M., Calado, A., Braga, J., Rino, J.,  Carvalho, C., Tavanez, J. P., Wahle, E., Rosa, A. C., Carmo- Fonseca, M.  (2002) The intranuclear mobility of messenger RNA binding proteins is  ATP-dependent and temperature- sensitive. J. Cell Biol. 159, 795-805.

Juge,  F., Zaessinger, S., Temme, C., Wahle, E., Simonelig, M. (2002) Control  of poly(A) polymerase level is essential to cytoplasmic polyadenylation  and early development in Drosophila. EMBO J. 21, 6603-6613.

Meyer, S., Urbanke, C., Wahle, E. (2002) Equilibrium studies on the association of the nuclear poly(A) binding protein with poly(A) of different lengths. Biochemistry 41, 6082-6089.

van  Dijk, E., Cougot, N., Meyer, S., Babajko, S., Wahle, E., Séraphin, B.  (2002) The human Dcp2 protein: a catalytically active mRNA decapping  enzyme localized in specific cytoplasmic structures. EMBO J. 21, 6915-6924.

2000

Dehlin, E., Wormington, M., Körner, C. G., Wahle, E. (2000) Cap-dependent deadenylation of mRNA. EMBO J., 19, 1079-1086

Calado, A., Kutay, U., Kühn, U., Wahle, E., Carmo-Fonseca, M. (2000) Deciphering the cellular pathway for transport of poly(A)-binding protein II. RNA, 6, 245-256

Keller,  R. W., Kühn, U., Aragon, M., Bornikova, L., Wahle, E., Bear, D. G.  (2000) The nuclear poly(A) binding protein, PABP2, forms an oligomeric  particle covering the length of the poly(A) tail. J. Mol. Biol., 297,  569-583

Winstall, E., Sadowski, M., Kühn, U., Wahle, E.,  Sachs, A. B. (2000) The Saccharomyces cerevisiae RNA binding protein  rbp29 functions in cytoplasmic mRNA metabolism. J. Biol. Chem. 275,  21817-21826

Calado, A., Tomè, F. M. S., Brais, B., Rouleau, G. A., Kühn, U., Wahle, E., Carmo-Fonseca,  M. (2000) Nuclear inclusions in oculopharyngeal muscular dystrophy  consist of poly(A) binding protein 2 aggregates which sequester poly(A)  RNA. Hum. Mol. Genet. 9, 2321-2328.

1999

Smith, J. J., Rücknagel, P., Schierhorn, A., Nemeth, A., Tang, J.,  Linder, M., Herschman, H. R., Wahle, E. (1999) Unusual sites of arginine  methylation in poly(A)-binding protein II and in vitro methylation by  protein arginine methyltransferases PRMT1 and PRMT3. J. Biol. Chem. 274,  13229-13234

Wahle, E., Rüegsegger, U. (1999) 3` end processing of pre-mRNA in eucaryotes. FEMS Microbiology Rev. 23, 277-295

Benoit,  B., Nemeth, A., Aulner, N., Kühn, U., Simonelig, M., Wahle, E.,  Bourbon, H.-M. (1999) The Drosophila poly(A) binding protein II is  ubiquitous throughout Drosophila development and has the same function  in mRNA polyadenylation as its bovine homolog in vitro. Nucleic Acids  Res. 27, 3771-3778

Buiting, K., Körner, C., Ulrich, B.,  Wahle, E., Horsthemke, B. (1999) The human gene for the poly(A)-specific  ribonuclease (PARN) maps to 16p13 and has a truncated copy in the  Prader-Willi/Angelman syndrome region on 15q11-q13. Cytogen. Cell Genet. 87, 125-131

1998

Körner, C. G., Wormington, M., Muckenthaler, M., Schneider, S., Dehlin,  E., Wahle, E. (1998) The deadenylating nuclease (DAN) is involved in  poly(A) tail removal during the meiotic maturation of Xenopus Oocytes.  EMBO J. 17, 5427-5437

1997

Wittmann, T., Wahle, E. (1997) Purification and characterization of full-length mammalian poly(A) polymerase. Biochim. Biophys. Acta 1350, 293-305

Wahle, E., Kühn, U. (1997) The mechanism of 3`-cleavage and polyadenylation of eucaryotic pre-mRNA. Progr. Nucleic Acid Res. Mol. Biol. 57, 41-71

Wahle,  E., Keller, W. (1997) Capping, methylation, and 3`-end formation of  messenger RNA precursors. In: A. Krainer (Herausgeber), Frontiers in  Molecular Biology: mRNA Processing, S. 280-309. IRL Press, Oxford

Körner,  C., Wahle, E. (1997) Poly(A) tail shortening by a mammalian  poly(A)-specific 3`-exoribonuclease. J. Biol. Chem. 272, 10448-10456

1996

Wahle, E., Keller, W. (1996) The biochemistry of polyadenylation. Trends Biochem. Sci. 21, 247-250

1995

Wahle, E. (1995) Poly(A) tail length control is caused by termination of processive synthesis. J. Biol. Chem. 270, 2800-2808

Wahle, E. (1995) 3`-end cleavage and polyadenylation of mRNA precursors. Biochim. Biophys. Acta 1261, 183-194

Nemeth,  A., Krause, S., Blank, D., Jenny, A., Jenö, P., Lustig, A., Wahle, E.  (1995) Isolation of genomic and cDNA clones encoding bovine poly(A)  binding protein II. Nucleic Acids Res. 23, 4034-4041

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