Martin Luther University Halle-Wittenberg

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Publications AG Molecular Enzymology

2008

Kluger, R. & Tittmann, K. (2008)
Thiamin Diphosphate Catalysis: Enzymic and Nonenzymic Covalent Intermediates.
Chemical Reviews 108, 1797-1833


Weidner, A., Neumann, P., Wille, G., Stubbs, M. T. & Tittmann, K. (2008)
Crystallization and preliminary X-ray diffraction analysis of full-length and proteolytically activated pyruvate oxidase from Escherichia coli. Acta Crystallographica Section F 64 (3), 179-181

Kaplun, A., Binshtein, E., Vyazmensky, M., Steinmetz, A., Barak, Z., Chipman, D., Tittmann, K., Shaanan, B. (2008)
Glyoxylate carboligase lacks the canonical active site glutamate of  thiamin-dependent enzymes.
Nature Chemical Biology 4 (2), 113-118


Werther, T., Spinka, M. Tittmann, K., Schütz, A., Golbik, R., Mrestani-Claus, C., Hübner, G. & König, S. (2008)
Amino acids allosterically regulate the thiamine diphosphate-dependent -keto acid decarboxylase from mycobacterium tuberculosis.
J. Biol. Chem. 283, 5344-5354

Meshalkina, L., Kochetov, G., Brauer, J., Hübner, G. Tittmann, K. & Golbik, R. (2008)
New evidence for cofactor’s amino group function in thiamine catalysis on the example of transketolase.
Biochem. Biophys. Res. Comm. 366 (3), 692-697


2007

Asztalos, P., Parthier, C., Golbik, R., Kleinschmidt, M., Hübner, G., Weiss, M., Friedemann, R., Wille, G. & Tittmann, K. (2007)
Strain and near attack conformers in enzymic thiamin catalysis: X-ray crystallographic snapshots of bacterial transketolase in covalent complex with donor ketoses xylulose 5-phosphate and fructose 6-phosphate, and in noncovalent complex with acceptor aldose ribose 5-phosphate.
Biochemistry 46, 12037-12052

Seifert, F., Ciszak, E., Korotchkina, L., Golbik, R., Spinka, M., Dominiak, P., Sidhu, S., Brauer, J., Patel, M.S. & Tittmann, K. (2007)
Phosphorylation of serine 264 impedes active site accessibility in the E1 component of the human pyruvate dehydrogenase multienzyme complex.
Biochemistry 46, 6277-6287

2006

Seifert, F., Golbik, R., Brauer, J., Lilie H., Schröder-Tittmann, K., Hinze, E.,
Korotchkina, L.G., Patel, M.S., and Tittmann, K. (2006)
Direct kinetic evidence for half-of-the-sites reactivity in E1 component of human pyruvate dehydrogenase multienzyme complex through alternating sites cofactor activation. Biochemistry 45, 12775-12785

Joseph, E., Wei, W., Tittmann, K., and Jordan, F. (2006)
Function  of a conserved loop of the beta-domain, not involved in thiamin diphosphate binding, in catalysis and substrate
activation in yeast pyruvate decarboxylase.
Biochemistry 45, 13517-13527

Wille, G., Meyer, D., Steinmetz, A., Hinze, E., Golbik, R., and Tittmann, K. (2006)
The catalytic cycle of a thiamin diphosphate enzyme examined by cryocrystallography.
Nature Chemical Biology 2 (6), 324-328

Hübner, G., Golbik, R., and Tittmann, K. (2006)
Hydrogen transfer in the action of thiamin diphosphate enzymes. In Handbook of hydrogen transfer in biological systems, vol. 4 (Eds.: R. L. Schowen, J. Klinman).

2005

Tittmann, K., Wille, G., Golbik, R., Weidner, A., Ghisla, S. & Hübner, G. (2005)
A radical phosphate transfer mechanism for the thiamin diphosphate- and FAD-dependent
pyruvate oxidase from Lactobacillus plantarum. Kinetic coupling of
intercofactor electron transfer with phosphate transfer via a transient FAD
semiquinone/ hydroxyethyl-ThDP radical pair.
Biochemistry 44, 13291-13303

Chipman, D. M., Duggleby, R. G. & Tittmann, K. (2005)
Mechanisms of Acetohydroxyacid Synthases. Curr. Opin. Chem. Biol. 9, 1-7.

Tittmann, K., Golbik, R., Neef, H., Hübner, G. & Kern, D. (2005)
Kinetic control of thiamin diphosphate activation in enzymes studied by proton nitrogen correlated NMR
spectroscopy.
Biochemistry 44, 8697-8700.

Tittmann, K. (2005)
Autopsie von Enzymvarianten. Aufklärung molekularer Details der
Enzymkatalyse.
BIOforum 6/2005, 2-4.

Nemeria, N., Tittmann, K., Ebenezer, J., Zhou, L., Vazquez-Coll, M., Arjunan, P.,
Hübner, G., Furey, W. & Jordan, F. (2005)
Glutamate 636 of the Escherichia coli pyruvate dehydrogenase multienzyme complex E1 component participates in active
center communication and protects from carboligase side reaction with pyruvate. An engineered acetolactate synthase with unusual stereoselectivity.
J. Biol. Chem. 280, 21473-21482.

Schütz, A., Golbik, R., König, S., Hübner, G. & Tittmann, K. (2005)
Intermediates and transition states in thiamin diphosphate-dependent decarboxylases. A kinetic and
NMR study on indolepyruvate decarboxylase using indolepyruvate, benzoylformate
and pyruvate as substrates.
Biochemistry 44, 6164-6179.

Vinogradov, M., Kaplun, A., Vyazmensky, M., Golbik, R., Tittmann, K.,
Uhlemann, K., Meshalkina, L., Barak, Z., Hübner, G. & Chipman, D. (2005)
Monitoring the acetohydroxyacid synthase reaction and related carboligations by
circular dichroism spectroscopy.
Analytical Biochemistry 342, 126-133.

Golbik, R., Meshalkina, L., Sandalova, T., Tittmann, K., Fiedler, E., Neef, H.,
König, S., Kluger, R., Kochetov, G., Schneider, G & Hübner, G. (2005)
Effect of coenzyme modification on structural and catalytic properties of transketolase
wildtype and the variant E418A from Saccharomyces cerevisiae. Contrasting
protonation state requirements of ThDP in decarboxylases and transketolases.
FEBS
Journal
272, 1326-1342.

Tittmann, K., Vyazmensky, M., Hübner, G., Barak, Z. & Chipman, D. (2005)
The carboligation reaction of acetohydroxyacid synthase II: Steady state intermediate
distribution in wild-type and mutants by NMR.
Proc. Natl. Acad. Sci. USA 102, 553-
558.

2004

Tittmann, K., Schröder, K., Golbik, R., McCourt, J., Duggleby, R., Barak, Z., Chipman, D. &
Hübner, G. (2004)
Electron transfer in acetohydroxyacid synthase as a side reaction
in catalysis. Implications for the reactivity and partitioning of the key intermediate
alpha-hydroxyethyl-thiamin diphosphate in a „nonredox“ flavoenzyme.

Biochemistry 43, 8652-8661.

Friedemann, R., Tittmann, K., Golbik, R., & Hübner, G. (2004)
Modelling of elementary steps in thiamin catalysis. Intl. J. Quant. Chem. 99, 109-114.

2003

Tittmann, K., Golbik, R., Uhlemann, K., Khailova, L., Schneider, G., Patel, M.,
Jordan, F., Chipman, D.M., Duggleby, R.G. & Hübner, G. (2003)
NMR analysis of covalent intermediates in thiamin diphosphate enzymes.
Biochemistry (Accelerated article) 42, 7885-7891.

Schütz, A., Golbik, R., Tittmann, K., Svergun, D.I., Koch, M.H.J., Hübner, G. &
König, S. (2003)
Studies on structure-function relationships of indolepyruvatedecarboxylase from Enterobacter cloacae,
a key enzyme of the indole acetic acid pathway.

European Journal of Biochemistry 270, 2322-2331.

Tittmann, K., Golbik, R. & Hübner, G. (2003)
Beobachtung von Enzymen bei der Arbeit. Untersuchung von Reaktionsmechanismen.
Scientia halensis 03/2003, 27-30.

2002

Tittmann, K., Golbik, R., Uhlemann, K., Khailova, L., Schneider, G., Patel, M.,
Jordan, F., Chipman, D.M., Duggleby, R.G. & Hübner, G. (2002)
How thiamin is working in enzymes: Time-resolved NMR snapshots of ThDP-dependent enzymes in
action.
In: Thiamin: Catalytic Mechanisms and Role in Normal and Disease States, pp
57-76.

Tittmann, K., Golbik, R., Ghisla, S., Hübner, G. (2002)
Phosphate mediates electron transfer in pyruvate oxidase. In: Flavins and Flavoproteins 2002 (Eds. N. Scrutton, R.
Perham and S. Chapman).

Tittmann, K., Hübner, G., Barak, Z., Chipman, D. (2002)
Acetohydroxyacid synthase catalyses electron transfer between a-hydroxyethyl-thiamin diphosphate and FAD in a
slow side reaction of catalysis.
In: Flavins and Flavoproteins 2002 (Eds. N. Scrutton,
R. Perham and S. Chapman).

2001

Bar-Ilan, A., Balan, V., Tittmann, K., Golbik, R., Vyazmensky, M., Hübner, G.,
Barak, Z., Chipman, D. (2001)
Binding and Activation of Thiamin Diphosphate in
Acetohydroxyacid Synthase.
Biochemistry 40, 11946-11954

Liu, M., Sergienko, EA., Guo, F., Wang, J., Tittmann, K., Hübner, G., Furey, W.,
Jordan, F. (2001)
Catalytic acid-base groups in yeast pyruvate decarboxylase. 1.
Site-directed mutagenesis and steady-state kinetic studies on the enzyme with the
D28A, H114F, H115F, and E477Q substitutions.
Biochemistry 40, 7355-7368

Wang, J., Golbik, R., Seliger, B., Spinka, M., Tittmann, K., Hübner, G., Jordan, F.
(2001)
Consequences of a modified putative substrate-activation site on catalysis by
yeast pyruvate decarboxylase.
Biochemistry 40, 1755-1763

Fiedler, E., Golbik, R., Schneider, G., Tittmann, K., Neef, H., König, S., Hübner,
G. (2001)
Examination of donor substrate conversion in yeast transketolase.
J. Biol. Chem. 276, 16051-16058

2000

Tittmann, K., Golbik, R., Ghisla, S. & Hübner, G. (2000)
Mechanism of Elementary Catalytic Steps in Pyruvate Oxidase from Lactobacillus plantarum. Biochemistry 39,
10747-10754.

1999

Tittmann, K., Golbik, R., Ghisla, S. & Hübner, G. (1999)
Single Step Analysis of Pyruvate Oxidase from Lactobacillus plantarum. Kinetics, Mechanism and Regulation.
Flavins and Flavoproteins 1999. (Eds. Ghisla, S.; Kroneck, P.; Macheroux, P.; Sund,
H.) Agency for scientific publications, Berlin, 395-400

1998

Tittmann, K., Mesch, K., Pohl, M. & Hübner, G. (1998)
Activation of Thiamine Diphosphate in Pyruvate Decarboxylase from Zymomonas mobilis.
FEBS Lett. 441, 404-406

Tittmann, K., Proske, D., Spinka, M., Ghisla, S., Rudolph, R., Hübner, G. & Kern,
G. (1998)
Activation of Thiamin Diphosphate and FAD in the Phosphate-Dependent
Pyruvate Oxidase from Lactobacillus plantarum.
J. Biol. Chem. 273, 12929-12934

Hübner, G., Tittmann, K., Killenberg-Jabs, M., Schäffner, J., Spinka, M., Neef,
H., Kern, D., Kern, G., Schneider, G., Wikner, C. & Ghisla, S. (1998)
Activation of ThDP in Enzymes. Biochim. Biophys. Acta 1385, 221-228

1997

Kern, D., Kern, G., Neef, H. Tittmann, K., KillenbergG-Jabs, M., Wikner, C.,
Schneider, G. & Hübner, G. (1997)
How Thiamine Diphosphate is Activated in Enzymes. Science 275, 67-70

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