Publikationen Abt. Technische Enzymologie
Publikationen der Arbeitsgruppe:
2017
G.-J. Krauß and Ulbrich-Hofmann, R. (2017) Zur Gründung des Instituts für Biochemie in Halle vor 50 Jahren. BIOspektrum 23, 721-723 [Abstract]
Dreßler, L. and Ulbrich-Hofmann, R. (2017) Phospholipase D Activity in Relation to the Size of Substrate Micelles. J. Am. Oil Chem. Soc. 94, 1253-1259 [Abstract]
Dreßler, L., Michel, M., Thondorf, I., Mansfeld, J., Golbik, R., and Ulbrich-Hofmann, R. (2017) Metal ions and phosphatidylinositol 4,5-bisphosphate as interacting effectors of alpha-type plant phospholipase D. Phytochemistry 138, 57-64 [Abstract ]
2016
Jablonicka, V., Mansfeld, J., Heilmann, I., Obložinský, M., and Heilmann, M. (2016) Identification of a secretory phospholipase A2 from Papaver somniferum L. that transforms membrane phospholipids. Phytochemistry 129, 4-13 [Abstract ]
Arnold, U., and Raines, R.T. (2016) Replacing a single atom accelerates the folding of a protein and increases its thermostability. Org. Biomol. Chem. 14, 6780-5 (Abstract )
Khatoon, H., Mansfeld, J., and Ulbrich-Hofmann, R. (2016) Phospholipid hydrolysis and transphosphatidylation by phospholipase D from indian mustard seeds in two-phase systems. J. Am. Oil Chem. Soc. 93, 365-72 (Abstract )
2015
Mansfeld, J. (2015) Microbial phospholipases. AOCS Lipid Library
Kölbel, K., Weininger, U., Ihling, C., Mrestani-Klaus, C., and Ulbrich-Hofmann, R. (2015) Native state dynamics affects the folding transition of porcine pancreatic phospholipase A2. Biophys. Chem. 206, 12-21 (Abstract )
Khatoon, H., Mansfeld, J., Schierhorn, A., and Ulbrich-Hofmann, R. (2015) "Purification, sequencing and characterization of phospholipase D from Indian mustard seeds." Phytochem. 117, 65–75 (Abstract )
Kiesgen, S., Arndt, M. A., Körber, C., Arnold, U., Weber, T., Halama, N., Keller, A., Bötticher, B., Schlegelmilch, A., Liebers, N., Cremer, M. Herold-Mende, C., Dyckhoff, G., Federspil, P. A., Jensen, A. D., Jäger, D., Kontermann, R. E., Mier, W., and Krauss, J. (2014) "An EGF receptor targeting Ranpirnase-diabody fusion protein mediates potent antitumour activity in vitro and in vivo." Cancer Lett. 357, 364-73 (Abstract)
2014
Kiesgen, S., Liebers, N., Cremer, M., Arnold, U., Weber, T., Keller, A., Herold-Mende, C., Dyckhoff, G., Jäger, D., Kontermann, R. E., Arndt, M. A. E., and Krauss, J. (2014) "A fusogenic dengue virus-derived peptide enhances antitumor efficacy of an antibody–ribonuclease fusion protein targeting the EGF receptor." Protein Eng. Des. Sel. 27, 331–338 (Abstract)
Lux, G., Mansfeld, J., and Ulbrich-Hofmann, R. (2014) "Phospholipase A2-catalyzed acylation of lysophospholipids analyzed by experimental design." Enzyme Microb. Technol. 64-65, 60-66 (Abstract)
Dressler, L., Golbik, R. and Ulbrich-Hofmann, R. (2014) "Lanthanides as substitutes for calcium ions in the activation of plant α-type phospholipase D." Biol. Chem. 395, 791–799 (Abstract)
Arnold, U. (2014) "Stability and folding of amphibian ribonuclease A superfamily members in comparison to mammalian homologues." FEBS J. 281, 3559-75 (Abstract)
Mansfeld, J., Schöpfel, M., Lorenz, J., Trutschel, T., Heilmann, I., Brandt, W., Ulbrich-Hofmann, R. (2014) "Probing selected structural regions in the secreted phospholipase A2 from Arabidopsis thaliana for their impact on stability and activity." Biochimie 101, 60-66 (Abstract )
Dippe, M., Dressler, L., and Ulbrich-Hofmann, R. (2014) "Fe(III)-resorcylate as a spectrophotometric probe for phospholipid–cation interactions." Anal. Biochem. 445, 54–59 (Abstract )
2013
Hacke, M., Gruber, T., Schulenburg, C., Balbach, J. and Arnold, U. (2013) "Consequences of proline-to-alanine substitutions for the stability and refolding of onconase." FEBS J. 280, 4454-4462 (Abstract)
Oblozinsky, M., Bezakova, L., Mansfeld, J., Heilmann, I., and Ulbrich-Hofmann, R. (2013) "Differences in the effect of phosphatidylinositol 4,5-bisphosphate on the hydrolytic and transphosphatidylation activities of membrane-bound phospholipase D from poppy seedlings." Plant Physiol. Biochem. 69, 39-42 (Abstract)
Solé, M., Brandt, W., and Arnold, U. (2013) "Striking stabilization of Rana catesbeiana ribonuclease 3 by guanidine hydrochloride." FEBS Lett. 587, 737-742 (Abstract)
Arnold, U., Huck, B. R., Gellman, S. H., and Raines, R. T. (2013) "Protein prosthesis: β-peptides as reverse-turn surrogates." Protein Sci. 22, 274-279 (Abstract)
Arnold, U. (2013) "Tumortherapeutika: Neue Perspektiven durch Ribonucleasen" GIT-labor ((Abstract)
Mansfeld, J. (2013) "Stearolysin" in Handbook of Proteolytic Enzymes 3rd ed., Rawlings, N. D.and Salvesen, G. S. (eds), Elsevier
2012
Arnold ,U., Huck, B. R., Gellman, S. H., and Raines, R.T. (2012) "Protein prosthesis: β-peptides as reverse-turn surrogates." Protein Sci. [Epub ahead of print]
Müller, A. O., Mrestani-Klaus, C., Schmidt, J., Ulbrich-Hofmann, R., and Dippe, M. (2012) "New cardiolipin analogs synthesized by phospholipase D-catalyzed transphosphatidylation." Chem. Phys. Lipids 165, 787-793. (Abstract)
Heimer, N. and Arnold, U. "Comparative characterization of the ribonuclease A homologue Rana catesbeiana ribonuclease 3."
Open J. Biol. Biochem. 2012, 1-3. (Abstract) , (Pdf)
Arnold, U. and Schöpfel, M (2012) "Significant stabilization of ribonuclease A by additive effects" FEBS J. 279, 2508-2519. (Abstract)
Dippe, M., Müller, Q. M., Sinz, A., and Ulbrich-Hofmann, R. (2012) "Phospholipases A1 from Armillaria ostoyae provide insight into the substrate recognition of alpha/beta-hydrolase fold enzymes." J. Am. Oil Chem. Soc. 89, 1435-1448. (Abstract)
2011
Schöpfel, M., Tziridis, A., Arnold, U., and Stubbs, M. (2011) "Towards a restriction proteinase: Construction of a self-activating enzyme." ChemBioChem 12, 1523-1527 (PubMed)
Dippe, M. and Ulbrich-Hofmann, R. (2011) "Phospholipid acylhydrolases trigger membrane degradation during fungal sporogenesis." Fungal Genet. Biol. 48, 921-927. (PubMed)
Mansfeld, J., Brandt, W., Haftendorn, R., Schöps, R., and Ulbrich-Hofmann, R. (2011) "Discrimination between the regioisomeric 1,2- and 1,3-diacylglycerophosphocholines by phospholipases." Chem. Phys. Lipids 164, 196-204 (PubMed )
Oblozinski, M., Bezakova, L., Mansfeld, J., and Ulbrich-Hofmann, R. (2011) "The transphosphatidylation potential of a membrane-bound phospholipase D from poppy seedlings." Phytochem. 72, 160-165 (PubMed)
Welker, S., Markert, Y., Köditz, J., Mansfeld, J., and Ulbrich-Hofmann, R. (2011) "Disulfide bonds of phospholipase A2 from bee venom yield discrete contributions to its conformational stability." Biochimie 93, 195-201 (PubMed)
Arnold, U., Leich, F., Neumann, P., Lilie, H., and Ulbrich-Hofmann, R. (2011) "Crystal structure of RNase A tandem enzymes and their interaction with the cytosolic ribonuclease inhibitor." FEBS J. 278, 331-340 (PubMed)
Watkins, R. W., Arnold, U., and Raines, R. T. (2011) "Ribonuclease S redux." Chem. Commun. (Camb) 47, 973-975 (PubMed)
2010
Arnold, U. (2010) "Stability and Stabilization of Proteins: The Ribonuclease A Example." in "Protein Folding", Walters, E. C. (Ed.), NOVA Publishers (Index)
Dippe, M. and Ulbrich-Hofmann, R. (2010) "A spectrophotometric microtiterplate assay to determine the transphosphatidylation potential of phospholipase D." J. Am. Oil Chem. Soc. 87, 1005-1011. (Abstract)
Schulenburg, C., Weininger, U., Neumann, P., Meiselbach, H., Stubbs, M. T., Sticht, H., Balbach, J., Ulbrich-Hofmann, R., and Arnold, U. (2010) "Impact of the C-terminal disulfide bond on the folding and stability of onconase." ChemBioChem 11, 978-986. (PubMed)
Dürrschmidt, P., Mansfeld, J., and Ulbrich-Hofmann, R. (2010) "Refolding of the non-specific neutral protease from Bacillus stearothermophilus proceeds via an autoproteolytically sensitive intermediate." Biophys. Chem. 147, 66-73. (PubMed)
2009
Schulenburg, C., Löw, C., Weininger, U., Mrestani-Klaus, C., Hofmann, H., Balbach, J., Ulbrich-Hofmann, R., and Arnold, U. (2009) "The folding pathway of Onconase is directed by a conserved intermediate." Biochemistry 48, 8449 - 8457 (PubMed)
Mrestani-Klaus, C., Kölbel, K. and Ulbrich-Hofmann, R. (2009) Thermodynamic stability and native-state dynamics of porcine pancreatic phospholipase A2 studied by 1H NMR spectroscopic measurements. Adv. Exp. Med. Biol. 611, 67-68. (No abstract)
Dippe, M. and Ulbrich-Hofmann, R. (2009) "Spectrophotometric determination of phosphatidic acid via iron(III) complexation for assaying phospholipase D activity." Anal. Biochem. 392, 169-173. (PubMed )
Mansfeld, J. (2009) "Plant phospholipases A2: Perspectives on biotechnological applications." Biotechnol Lett. 31, 1373-1380 (PubMed )
Arnold, U. (2009) "Incorporation of non-natural modules into proteins: Structural features beyond the genetic code." Biotechnol Lett. 31, 1129-1139. (PubMed )
Mansfeld, J. and Ulbrich-Hofmann, R. (2009) "The modulation of phospholipase D activity in vitro." Biochim. Biophys. Acta 1791, 913 - 926. (PubMed )
Dippe, M. and Ulbrich-Hofmann, R. (2009) "Substrate specificity in phospholipid transformations by plant phospholipase D isoenzymes." Phytochemistry 70, 361–365. (PubMed )
Pecher, P. and Arnold, U. (2009) "The effect of additional disulfide bonds on the stability and folding of ribonuclease A." Biophys. Chem. 141, 21-28. (PubMed )
Hofmann, H., Weininger, U., Löw, C., Golbik, R., Balbach, J., and Ulbrich-Hofmann, R. (2009) "Fast amide proton exchange reveals close relation between native-state dynamics and unfolding kinetics." J. Am. Chem. Soc. 131, 140-146. (PubMed)
2008
Kuppe, K., Kerth, A., Blume, A., and Ulbrich-Hofmann, R. (2008) "Calcium-induced membrane microdomains trigger plant phospholipase D activity." ChemBioChem 9, 2853-2859. (PubMed)
Tripathi, P., Hofmann, H., Kayastha, A. M., and Ulbrich-Hofmann R. (2008) "Conformational stability and integrity of alpha-amylase from mung beans: Evidence of kinetic intermediate in GdmCl-induced unfolding." Biophys. Chem. 137, 95-98. (PubMed)
Screening for nutritive peptides that modify cholesterol 7alpha-hydroxylase expression." J. Agric. Food Chem. 56, 4987-4994. (PubMed)
Dippe, M., Mrestani-Klaus, C., Schierhorn, A., and Ulbrich-Hofmann, R. (2008) "Phospholipase D-catalyzed synthesis of new phospholipids with polar head groups." Chem. Phys. Lipids 152, 71-77. (PubMed)
Arnold, U. (2008) "Aspects of the cytotoxic action of ribonucleases." Curr. Pharm. Biotechnol. 9, 161-168. (Abstract) (Editorial)
Hofmann, H., Golbik, R., Ott, M., Hübner, C. G., and Ulbrich-Hofmann, R. (2008) "Coulomb forces control the density of the collapsed unfolded state of barstar." J. Mol. Biol. 376, 597-605. (PubMed)
2007
2007) "Identification of three phases in Onconase refolding." FEBS J. 274, 5826-5833. (PubMed )
Protein prosthesis: 1,5-disubstituted[1,2,3]triazoles as cis-peptide bond surrogates. J. Am. Chem. Soc. 129, 12670-12671. (PubMed )
Schmelzer, C. E. H., Schöps, R., Reynell, L., Ulbrich-Hofmann, R., Neubert, R. H. H., Raith, K. (2007) "Peptic digestion of β-casein.Time course and fate of possible bioactive peptides." J. Chromatogr. A 1166, 108-115. (PubMed )
Leich, F., Stöhr, N., Rietz, A., Ulbrich-Hofmann, R., and Arnold, U. (2007) "Endocytotic internalization as a crucial factor for the cytotoxicity of ribonucleases." J. Biol. Chem. 282, 27640-27646. (PubMed)
Mansfeld, J. and Ulbrich-Hofmann, R. (2007) "Secretory phospholipase A2-alpha from Arabidopsis thaliana: functional parameters and substrate preferences." Chem. Phys. Lipids 150, 156-166. (PubMed)
Schulenburg, C., Ardelt, B., Ardelt, W., Arnold, U., Shogen, K., Ulbrich-Hofmann, R., and Darzynkiewicz, Z. (2007) "The interdependence between catalytic activity, conformational stability, and cytotoxicity of onconase." Cancer Biol Ther. 6, 1233-1239. (PubMed)
Tripathi, P., Lo Leggio, L., Mansfeld, J., Ulbrich-Hofmann, R., and Kayastha, A. M. (2007) "α-Amylase from mung beans (Vigna radiata) - Correlation of biochemical properties and tertiary structure by homology modelling." Phytochemistry 68, 1623-1631. (PubMed)
Stumpe, S., König, S., and Ulbrich-Hofmann, R. (2007) "Insights into the structure of plant alpha-type phospholipase D." FEBS J. 274, 2630–2640. (PubMed)
Markert, Y., Mansfeld, J., Schierhorn, A., Rücknagel, K. P., and Ulbrich-Hofmann, R. (2007) "Production of synthetically created phospholipase A2 variants with industrial impact." Biotechnol. Bioeng. 98, 48-59. (PubMed)
Mansfeld, J. and Ulbrich-Hofmann, R. (2007) "The stability of engineered thermostable neutral proteases from Bacillus stearothermophilus in organic solvents and detergents." Biotechnol. Bioeng. 97, 672-679. (PubMed)
2006
Arnold, U. and Ulbrich-Hofmann, R. (2006) "Natural and engineered ribonucleases as potential cancer therapeutics." Biotechnol. Lett. 28, 1615-1622. (PubMed)
Younus, H., Ulbrich-Hofmann, R., and Saleemuddin, M. (2006) "Inhibition of pancreatic ribonuclease A aggregation by antibodies raised against the native enzyme and its N-terminal dodecapeptide." Protein Pept. Lett. 13, 673-677. (PubMed)
Lerchner, A., Mansfeld, J., Kuppe, K., and Ulbrich-Hofmann, R. (2006) "Probing conserved amino acids in phospholipase D (Brassica oleracea var. capitata) for their importance in hydrolysis and transphosphatidylation activity." Protein Eng. Des. Sel. 19, 443-452. (PubMed)
Mansfeld, J., Gebauer, S., Dathe, K., and Ulbrich-Hofmann, R. (2006) "Secretory phospholipase A2 from Arabidopsis thaliana: Insights into the three-dimensional structure and the amino acids involved in catalysis." Biochemistry 45, 5687-5694. (PubMed) , (Supporting information)
Leich, F., Köditz, J., Ulbrich-Hofmann, R., and Arnold, U., (2006) "Tandemization endows bovine pancreatic ribonuclease with cytotoxic activity.." J. Mol. Biol. 19, 1305-1313. (PubMed)
Arnold, U., Schulenburg, C., Schmidt, D., and Ulbrich-Hofmann, R. (2006) "Contribution of structural peculiarities of onconase to its high stability and folding kinetics." Biochemistry 45, 3580-3587. (PubMed)
2005
Lerchner, A., Mansfeld, J., Schäffner, I., Schoeps, R., Beer, H. K., and Ulbrich-Hofmann, R. (2005) "Two highly homologous phospholipase D isoenzymes from Papaver somniferum L. with different transphosphatidylation potential" Biochim. Biophys. Acta 1737, 94-101. (PubMed)
Ulbrich-Hofmann, R., Lerchner, A., Oblozinsky, M., and Bezakova, L. (2005) "Phospholipase D and its application in biocatalysis", Biotechnol. Lett. 27, 535-543. (PubMed)
Arnold, U., Köditz, J., Markert, Y., and Ulbrich-Hofmann, R. (2005) "Local fluctuations vs. global unfolding of proteins investigated by limited proteolysis" Biocatal. Biotrans. 23, 159-167 (Abstract)
Dürrschmidt, P., Mansfeld, J., and Ulbrich-Hofmann, R. (2005) "An engineered disulfide bridge mimics the effect of calcium to protect neutral protease against local unfolding", FEBS J. 272, 1523-1534. (PubMed)
Oblozinsky, M., Ulbrich-Hofmann, R., and Bezakova, L. (2005) "Head group specificity of phospholipase D isoenzymes from poppy seedlings (Papaver somniferum L.)", Biotechnol Lett. 27, 181-185. (PubMed)
Mansfeld, J., Petermann, E., Dürrschmidt, P., and Ulbrich-Hofmann, R. (2005) "The propeptide is not required to produce catalytically active neutral protease from Bacillus stearothermophilus", Protein Expr. Purif. 39, 219-228. (PubMed)
Ringseis, R., Matthes, B., Lehmann, V., Becker, K., Schöps, R., Ulbrich-Hofmann, R., and Eder, K. (2005) "Peptides and hydrolysates from casein and soy protein modulate the release of vasoactive substances from human aortic endothelial cells", Biochim. Biophys. Acta 1721, 89 - 97. (PubMed)
2004
Schmelzer, C. E. H., Schöps, R., Ulbrich-Hofmann, R., Neubert, R. H. H., and Raith, K. (2004) "Mass spectrometric characterization of peptides derived by peptic cleavage of bovine beta-casein", J. Chromatogr. A 1055, 87-92. (PubMed)
Köditz, J., Ulbrich-Hofmann, R., and Arnold, U. (2004) "Probing the unfolding region of ribonuclease A by site-directed mutagenesis", Eur. J. Biochem. 271, 4147-4156. (PubMed)
"Behaviour of a recombinant cabbage (Brassica oleracea) phospholipase D immobilized on CNBr-activated and antibody supports." Biotechnol. Appl.
2003
Markert, Y., Köditz, J., Ulbrich-Hofmann, R., and Arnold, U. (2003) "Proline versus charge concept for protein stabilization against proteolytic attack", Protein Eng. 16, 1041-1046. (PubMed)
Younus, H., Schöps, R., Lerchner, A., Rücknagel, K.-P., Schierhorn, A., Saleemuddin, M., and Ulbrich-Hofmann, R. (2003) "Proteolytic sensitivity of a recombinant phospholipase D from cabbage: Identification of loop regions and conformational changes", J. Protein Chem. 22, 499-508. (PubMed)
Ulbrich-Hofmann, R. (2003) "Enzyme-catalysed transphosphatidylation", Eur. J. Lipid Sci. Technol. 105, 305-307. Arnold, U., Hinderaker, M. P., Köditz, J., Golbik, R., Ulbrich-Hofmann, R., and Raines, R. T. (2003) "Protein Prosthesis: A Nonnatural Residue Accelerates Folding and Increases Stability", J. Am. Chem. Soc. 125, 7500-7501. (PubMed) (Supporting information)
Oblozinsky, M., Schöps, R., Ulbrich-Hofmann, R., and Bezakova, L. (2003) "Two uncommon phospholipase D isoenzymes from poppy seedlings (Papaver somniferum L.)", Biochim. Biophys. Acta 1631, 153-159. (PubMed)
2002
Schäffner, I., Rücknagel, K.-P., Mansfeld, J., and Ulbrich-Hofmann, R. (2002) "Genomic structure, cloning and expression of two phospholipase D isoenzymes from white cabbage", Eur. J. Lipid Sci. Technol. 104, 79-87. (Abstract)
Schöps, R., Schierhorn, A., Schäffner, I., Mansfeld, J., and Ulbrich-Hofmann, R. (2002) "Identification of phospholipase D from cabbage as N-terminally acetylated PLD2", J. Protein Chem. 21, 407-411. (PubMed)
Ulbrich-Hofmann, R., Mansfeld, J., Arnold, U., Köditz, J., Dürrschmidt, P., Markert, Y. (2002) "Lokal contra global - Strukturloops als Determinanten der Proteinstabilität", BIOforum 25, 14-17.
Younus, H., Köditz, J.,Saleemuddin, M., and Ulbrich-Hofmann, R. (2002) "Selective stabilization of a labile mutant form of bovine pancreatic ribonuclease A by antibodies", Biotechnol. Lett. 24, 1821-1826.
Arnold, U., Hinderaker, M. P., Nilsson, B. L., Huck, B. R., Gellman, S. H. and Raines, R. T. (2002) "Protein prosthesis: A semisynthetic enzyme with a ß-peptide reverse turn", J. Am. Chem. Soc. 124, 8522-8523. (PubMed) (Supporting information)
Arnold, U., Hinderaker, M. P., and Raines, R. T. (2002) "Semisynthesis of ribonuclease A using intein-mediated protein ligation", TheScientificWorldJOURNAL 2, 1823-1827. (PubMed)
Kühn, D., Dürrschmidt, P., Mansfeld, J., and Ulbrich-Hofmann, R. (2002) "Boilysin and thermolysin in dipeptide synthesis: a comparative study", Biotechnol. Appl. Biochem. 36, 71-76. (PubMed)
Köditz, J., Arnold, U., and Ulbrich-Hofmann, R. (2002) "Dissecting the effect of trifluoroethanol on ribonuclease A - Subtle structural changes detected by nonspecific proteases", Eur. J. Biochem. 269, 3831-3837. (PubMed)
Haftendorn, R. and Ulbrich-Hofmann, R. (2002) "Activity of phospholipase C in two-phase systems", Anal. Biochem. 306, 144-147. (PubMed)
Köditz, J. and Ulbrich-Hofmann, R. (2002) "pH-Stat titration allows the continuous determination of ribonuclease A activity toward cytidine 2`,3`-cyclic monophosphate at high substrate concentrations", Anal. Biochem. 305, 281-284. (PubMed)
Aurich, I., Dürrschmidt, P., Schierhorn, A., and Ulbrich-Hofmann, R. (2002) "Production of octadecylphospho-L-serine by phospholipase D", Biotechnol. Lett. 24, 585-590.
2001
Dittrich, N., and Ulbrich-Hofmann, R. (2001) "Transphosphatidylation by immobilized phospholipase D in aqueous media", Biotechnol. Appl. Biochem. 34, 189-194. (PubMed)
Markert, Y., Köditz, J., Mansfeld, J., Arnold, U., and Ulbrich-Hofmann, R. (2001) "Increased proteolytic resistance of ribonuclease A by protein engineering", Protein Eng. 14, 791-796. (PubMed)
Dürrschmidt, P., Mansfeld, J., and Ulbrich-Hofmann, R. (2001) "Differentiation between conformational and autoproteolytic stability of the neutral protease from Bacillus stearothermophilus containing an engineered disulfide bond ", Eur. J. Biochem. 268, 3612-3618. (PubMed)
Arnold, U., and Ulbrich-Hofmann, R. (2001) "Proteolytic degradation of ribonuclease A in the pretransition region of thermally and urea-induced unfolding", Eur. J. Biochem. 268, 93-97. (PubMed)
2000
Mansfeld, J., and Ulbrich-Hofmann, R. (2000) "Site-specific and random immobilization of thermolysin-like proteases reflected in the thermal inactivation kinetics", Biotechnol. Appl. Biochem. 32, 189-195. (PubMed)
Arnold, U., and Ulbrich-Hofmann, R. (2000) "Differences in the denaturation behavior of ribonuclease A induced by temperature and guanidine hydrochloride", J. Prot. Chem. 19, 345-352. (PubMed)
Hirche, F., and Ulbrich-Hofmann, R. (2000) "The interdependence of solvent, acceptor alcohol and enzyme source in transphosphatidylation by phospholipase D", Biocatal. Biotrans. 18, 343-353.
Ulbrich-Hofmann, R. (2000) "Phospholipases used in lipid transformations", in "Enzymes in Lipid Modification" (Bornscheuer, U. T., ed.) Wiley-VCH, Weinheim, N. Y., Chichester, Brisbane, Singapore, Toronto, pp. 219-262.
Haftendorn, R., Schwarze, G., and Ulbrich-Hofmann, R. (2000) "1,3-Diacylglycero-2-phosphocholines - Synthesis, aggregation behaviour and properties as inhibitors of phospholipase D", Chem. Phys. Lipids 104, 57-66. (PubMed)
1999
Ulbrich-Hofmann, R., Arnold, U., and Mansfeld, J. (1999) "The concept of the unfolding region for approaching the mechanisms of enzyme stabilization", J. Mol. Cat. B7, 125-131.
Van den Burg, B., de Kreij, A., Van der Veek, P., Mansfeld, J., and Venema, G. (1999) "Characterization of a novel stable biocatalyst obtained by protein engineering", Biotechnol. Appl. Biochem.30, 35-40. (PubMed)
Arnold, U. and Ulbrich-Hofmann, R. (1999) "Quantitative protein precipitation from guanidine hydrochloride-containing solutions by sodium deoxycholate/trichloroacetic acid", Anal. Biochem. 271, 197-199. (PubMed)
Mansfeld, J., Vriend, G., Van den Burg, B., Eijsink, V. G. H., and Ulbrich-Hofmann, R. (1999) "Probing the unfolding region in a thermolysin-like protease by site-specific immobilization", Biochemistry 38, 8240-8245. (PubMed)
Aurich, I., Hirche, F., and Ulbrich-Hofmann, R. (1999) "The determination of phospholipase D activity in emulsion systems", Anal. Biochem. 268, 337-342. (PubMed)
Hirche, F., and Ulbrich-Hofmann, R. (1999) "The interfacial pressure is an important parameter for the rate of phospholipase D catalyzed reactions in emulsion systems", Biochim. Biophys. Acta 1436, 383-389. (PubMed)
Arnold, U., Schierhorn, A., Ulbrich-Hofmann, R. (1999) "Modification of the unfolding region in bovine pancreatic ribonuclease and its influence on the thermal stability and proteolytic fragmentation", Eur. J. Biochem. 259, 470-475. (PubMed)
1998
Vagina, O., Gellerich, F. N., and Ulbrich-Hofmann, R. (1998) "The effect of headecylphosphocholine on the degradation of mitochondrial phospholipids", Mol. Cell. Biochem.183, 169-173. (PubMed)
Dittrich, N., Haftendorn, R., and Ulbrich-Hofmann, R. (1998)"Hexadecylphosphocholine and 2-modified 1,3-diacylglycerols as effectors of phospholipase D", Biochim. Biophys. Acta 1391, 265-272. (PubMed)
Pannenberg, I., Mansfeld, J., Ulbrich-Hofmann, R. (1998) "Identification of two isoenzymes (Accession nos. AF090444 and AF090445) of Phospholipase D from cabbage (Brassica oleracea var. capitata", Plant Physiol. 118, 1102.
Arnold, U., Schierhorn, A., Ulbrich-Hofmann, R. (1998) "Influence of the carbohydrate moiety on the proteolytic cleavage sites in ribonuclease B", J. Protein Chem. 17, 397-405. (PubMed)
Mansfeld, J., Vriend, G., Van den Burg, B., Venema, G.,Eijsink, V.G.H.,Ulbrich-Hofmann, R. (1998) "The effect of site-specific immobilization on the thermal stability of thermolysin-like neutral proteases", Progr. Biotechnol. 15, 513-516. (Abstract)
Arnold, U., Ulbrich-Hofmann, R. (1998) "Modification of the carbohydrate moiety in ribonuclease B and its influence on the protein stability probed by limited proteolysis", Progr. Biotechnol. 15, 121–126 (Abstract)
1997
Mansfeld, J., and Dautzenberg, H. (1997) "Coimmobilization of enzymes and cells in polyelectrolyte complex capsules", In: Methods in Biotechnol., Immobilization of Enzymes and Cells (G. F. Bickerstaff, ed.), Humana Press, New Jersey pp. 319-326.
Mansfeld, J., and Dautzenberg, H. (1997) "Immobilization of cells in polyelectrolyte complex capsules", In: Methods in Biotechnol., Immobilization of Enzymes and Cells (G. F. Bickerstaff, ed.), Humana Press, New Jersey pp. 309-318.
Becker, M., Spohn, U., and Ulbrich-Hofmann, R. (1997) "Detection and characterization of phospholipase D by flow injection analysis", Anal. Biochem. 244, 55-61. (PubMed)
Mansfeld, J., Vriend, G., Dijkstra, B. W., Veltman, O. R., Van den Burg, B., Venema, G., Ulbrich-Hofmann R., and Eijsink, V. G. H. (1997) "Extreme Stabilization of a thermolysin-like protease by an engineered disulfide bond", J. Biol. Chem. 272, 11152-11156. (PubMed)
Veltman, O. R., Vriend, G., Hardy, F., Mansfeld, J., Van den Burg, B., Venema, G., and Eijsink, V. G. H. (1997) "Mutational analysis of a surface area that is critical for the thermal stability of thermolysin-like proteases", Eur. J. Biochem. 248, 433-440. (PubMed)
Arnold, U., and Ulbrich-Hofmann, R. (1997) "Kinetic and thermodynamic thermal stabilities of ribonuclease A and ribonuclease B", Biochemistry 36, 2166-2172. (PubMed)
Hirche, F., Koch, M. H. J., König, S., Wadewitz, T., and Ulbrich-Hofmann, R. (1997) "The influence of organic solvents on phospholipid transformation by phospholipase D in emulsion systems", Enzyme Microb. Technol. 20, 453-461.
Hirche, F., Schierhorn, A., Scherer, G., and Ulbrich-Hofmann, R. (1997) "Enzymatic introduction of N heterocyclic and As containing head groups into glycerophospholipids", Tetrahedron Lett. 38, 1369-1370.
Aurich, I., Dürrschmidt, P., Hirche, F., and Ulbrich-Hofmann, R. (1997) "Transesterification of alkylphosphate esters by phospholipase D", Biotechnol. Lett. 19, 875-879.