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Publikationen AG Bordusa

Schriftenverzeichnis Original-Publikationen in referierten Journalen

  • Christoph Wiedemann, Günter      Hempel and Frank Bordusa. Reorientation dynamics and ion diffusivity of      neat dimethylimidazolium dimethylphosphate probed by NMR spectroscopy;      Published on 04 November 2019; RSC Advances;   
  • Sandra Liebscher and Frank Bordusa. Site-Specific Modification of Proteins via      Trypsiligase. In: Massa S., Devoogdt N. (eds) Bioconjugation. Methods in      Molecular Biology, vol 2033. Humana, New York, NY; First online: Springer      Protocol 23 July 2019;   
  • Sandra Liebscher and Frank Bordusa. Trypsiligase-Catalyzed Peptide and Protein      Ligation. In: Nuijens T., Schmidt M. (eds) Enzyme-Mediated Ligation      Methods. Methods in Molecular Biology, vol 2012. Humana, New York, NY;      First online: Springer Protocol 04 June 2019;   
  • Andreas H. Simon, Sandra Liebscher, Tobias H.      Aumüller, Dennis Treblow and Frank      Bordusa. Application of a Dual Internally Quenched Fluorogenic      Substrate in Screening for D-Arginine Specific Proteases; Front.      Microbiol., 03 April 2019;   
  • C. Meyer, S. Liebscher, F. Bordusa, Selective Coupling of Click Anchors to Proteins      via Trypsiligase, Bioconjugate Chemistry, Publication Date (Web):      December 15, 2015, DOI: 10.1021/acs.bioconjchem.5b00618.
  • A. Richard, C. Mrestani-Klaus, F. Bordusa. Impact of Ionic Liquids on the Structure of      Peptides Proved by HR-MAS NMR Spectroscopy, J. Mol. Liq.,    (2014).
  • N. Wehofsky, A. Pech, S. Liebscher, S. Schmidt,      H. Komeda, Y. Asano, F. Bordusa, D-Amino Acid Specific Proteases      and Native all-L-Proteins: A Convenient Combination for Semisynthesis, Angew.      Chem. Int. Ed, 47, 5456-5460 (2008).
  • Protein Folding Handbook, Eds.:      J. Buchner and T. Kiefhaber, Wiley-VCH, Weinheim in: Nachrichten aus der      Chemie, 09, 945-946 (2005).
  • F. Bordusa. Enzymatic      Reactions in Ionic Liquids, ChemFiles, 6, 13 (2005).
  • F. Bordusa. "Enzymatic Formation of C-N Bonds", In: Bioorganic Chemistry      Highlights II: From Chemistry to Biology, (C. Schmuck, H. Wennemers,      Hrsg.), WILEY-VCH Verlag, Weinheim, Germany, (2004).
  • F. Bordusa.      "Clostripain", In: Handbook of Proteolytic Enzymes, Second Edition      (A. J. Barrett; N. Rawlings & F. Woessner, Hrsg.), Elsevier, London,      pp. 1317-1319 (2004).
  • Peptide Arrays on Membrane      Supports: Synthesis and Applications, Eds.: J. Koch, M. Mahler,      Springer-Verlag Berlin Heidelberg 2002 in: ChemBioChem, 4, 544-546 (2003).
  • F. Bordusa.      Proteases in Organic Synthesis, Chemical Reviews, 102, 4817-4867      (2002).
  • F. Bordusa.      Substrate Mimetics in Protease Catalysis: Characteristics, Kinetics, and      Synthetic Utility, Current Protein and Peptide Science, 3, 159-180      (2002).
  • K. Rall & F. Bordusa. Substrate      mimetics-specific peptide ligases: Studies on the synthetic utility of a      zymogen and zymogen-like enzymes. J. Org. Chem., 67, 9103-9106 (2002).
  • F. Bordusa & H.-D. Jakubke. "Enzymatic synthesis", In: Houben-Weyl      E 22a "Synthesis of Peptides and Peptidomimetics", (M. Goodman,      A. Felix, L. Moroder & C. Toniolo, Hrsg.), Georg-Thieme Verlag,      Stuttgart, New York, pp. 642-664 (2001).
  • F. Bordusa.      Enzymes for peptide cyclization. ChemBioChem, 2, 405-409 (2001).
  • F. Bordusa.      Non-conventional CN-ligation catalysis: Programming of enzyme specificity      by substrate mimetics. Braz. J. Med. Bio. Res., 33, 469-485 (2000).
  • N. Wehofsky, M. Haensler, S. W.      Kirbach, J.-D. Wissmann & F. Bordusa. Effect      on freezing on the enzymatic coupling of specific amino acid-containing      fragments. Tetrahedron:      Asymmetry, 11,      2421-2428 (2000).
  • R. Günther & F. Bordusa. Substrate      mimetic-mediated protease catalysis: A novel approach to the synthesis of      carboxylic acid amides. Chemical biology, [Paper alert], Current      Opinion in Chemical Biology, 4, 243-251 (2000).
  • F. Bordusa, C. Dahl, H.-D. Jakubke, K.      Burger & B. Koksch. Synthesis of a-fluoroalkyl      substituted peptides via enzymatic fragment condensation. Tetrahedron: Asymmetry, 10, 307-313 (1999).
  • D. Ullmann & F. Bordusa.      "Clostripain", In "Handbook of Proteolytic Enzymes"      (A. J. Barrett; N. Rawlings & F. Woessner, Hrsg.), Academic Press,      London, pp. 759-762 (1998).
  • F. Bordusa, D. Ullmann, C. Elsner &      H.-D. Jakubke. Substrate mimetics      mediated peptide synthesis: an irreversible ligation strategy that is      independent of substrate specificity. Angew. Chem. Int. Ed., 36, 2473-2475 (1997).
  • F. Bordusa, D. Ullmann & H.-D.      Jakubke. Peptide synthesis from N- to C-terminus: an      advantageous strategy using protease catalysis. Angew. Chem. Int. Ed., 36, 1099-1101 (1997).
  • D. Ullmann, F. Bordusa,      K. Salchert & H.-D. Jakubke. A new simple route to the      synthesis of protease substrates in ice. Tetrahedron: Asymmetry, 7, 2047-2054 (1996).


1)     Verfahren zur Herstellung von Säureamiden
Aktenzeichen: Aktenzeichen DE 19834308.6

Erfinder: Dr. Frank Bordusa, Prof. Dr. H.-D. Jakubke
Anmelder: Universität Leipzig

2)     Verfahren zur Synthese von Peptiden, Peptidmimetika und Proteinen
Aktenzeichen DE10049673.3; WO0231177; US2004034197; EP1326995

Erfinder: Dr. Frank Bordusa, Prof. Dr. H.-D. Jakubke
Anmelder: Roche Diagnostics GmbH, Universität Leipzig

3)     Verfahren zur selektiven biokatalytischen Modifizierung von Peptiden und Proteinen Aktenzeichen DE10047857.3; PCT/EP01/11035

Erfinder: Dr. Frank Bordusa, Prof. Dr. H.-D. Jakubke
Anmelder: Roche Diagnostics GmbH, Universität Leipzig

4)     Verfahren zur Synthese von all-D-Peptiden
Aktenzeichen DE 10156274.8; EP 01127237.4; WO03047743; AU2002352044

Erfinder: Dr. Frank Bordusa, Dr. Sven Klussmann, Dr. Jens Burmeister
Anmelder: Noxxon Pharma AG

5)     Verfahren zur Synthese und selektiven Biokatalytischen Modifizierung von Peptiden, Peptidmimetika und Proteinen

Aktenzeichen DE 10240098.9-43; WO2004024751; US2006149035; EP1534734; CA2495111; MXPA05001917

Erfinder: Dr. Frank Bordusa, Prof. Dr. Rainer Rudolph, Dr. Nicole Wehofsky

Anmelder: Garching Innovation GmbH, Prof. Dr. Rainer Rudolph

6)  C-Terminal Modification of Polypeptides

Erfinder: Dr. Frank Bordusa, Dr. Eva Höß

Aktenzeichen DE 04 019 237.9; WO2006015879; EP1778839

Anmelder: Roche Diagnostics GmbH

1: Liebscher S, Kornberger P, Fink G, Trost-Gross  EM, Höss E, Skerra A, BordusaF. Derivatization of antibody fab  fragments: a designer enzyme for native proteinmodification.  Chembiochem. 2014 May 26;15(8):1096-100. doi:10.1002/cbic.201400059.    Epub 2014 Apr 29. PubMed PMID: 24782039.

2: Liebscher S, Schöpfel M, Aumüller T, Sharkhuukhen A, Pech A, Höss E, Parthier C, Jahreis G, Stubbs MT, Bordusa F. N-terminal protein modification bysubstrate-activated reverse proteolysis. Angew Chem Int Ed Engl. 2014 Mar10;53(11):3024-8. doi: 10.1002/anie.201307736. Epub 2014 Feb 12. PubMed PMID:24520050.

3:  Franke L, Liebscher S, Bordusa F. Engineering the oxyanion hole of  trypsin forpromoting the reverse of proteolysis. J Pept Sci. 2013 Dec  20.doi:10.1002/psc.2597.    [Epub ahead of print] PubMed PMID: 24357225.

4: Tietze AA, Bordusa F, Giernoth R, Imhof D, Lenzer T, Maass A, Mrestani-KlausC, Neundorf I, Oum K, Reith D, Stark A. On the nature of interactions betweenionic liquids and small amino-acid-based biomolecules. Chemphyschem. 2013 Dec16;14(18):4044-64. doi: 10.1002/cphc.201300736. Epub 2013 Nov 12. Review. PubMed PMID: 24222640.

5: Mrestani-Klaus C, Richardt A, Wespe C, Stark A, Humpfer E, Bordusa F.Structural studies on ionic liquid/water/peptide systems by HR-MAS NMRspectroscopy. Chemphyschem. 2012 May 14;13(7):1836-44.doi:10.1002/cphc.201100927.    Epub 2012 Apr 5. PubMed PMID: 22488934.

6:  Malesević M, Poehlmann A, Hernandez Alvarez B, Diessner A, Träger M,  RahfeldJU, Jahreis G, Liebscher S, Bordusa F, Fischer G, Lücke C. The  protein-free IANUSpeptide array uncovers interaction sites between  Escherichia coli parvulin 10 andalkyl hydroperoxide reductase.  Biochemistry. 2010 Oct 5;49(39):8626-35. doi:10.1021/bi101015p.   Epub 2010 Sep 9. PubMed PMID: 20806779.

7:  Wehofsky N, Wespe C, Cerovsky V, Pech A, Hoess E, Rudolph R, Bordusa F.  Ionic liquids and proteases: a clean alliance for semisynthesis.  Chembiochem. 2008 Jun 16;9(9):1493-9. doi: 10.1002/cbic.200800025. PubMed PMID: 18509837.

8:  Sangoro J, Iacob C, Serghei A, Naumov S, Galvosas P, Kärger J, Wespe  C,Bordusa F, Stoppa A, Hunger J, Buchner R, Kremer F. Electrical  conductivity andtranslational diffusion in the 1-butyl-3-methylimidazolium tetrafluoroborateionic liquid. J Chem Phys. 2008 Jun 7;128(21):214509. doi: 10.1063/1.2921796.PubMed PMID: 18537435.

9:  Sangoro JR, Serghei A, Naumov S, Galvosas P, Kärger J, Wespe C, Bordusa  F,Kremer F. Charge transport and mass transport in imidazolium-based ionic liquids.Phys Rev E Stat Nonlin Soft Matter Phys. 2008 May;77(5 Pt 1):051202. Epub 2008May 2. PubMed PMID: 18643055.

10:  Lodderstedt G, Sachs R, Faust J, Bordusa F, Kühn U, Golbik R, Kerth A,  Wahle E, Balbach J, Schwarz E. Hofmeister salts and potential  therapeutic compoundsaccelerate in vitro fibril formation of the N-terminal domain of PABPN1containing a disease-causing alanine extension. Biochemistry. 2008 Feb19;47(7):2181-9. doi: 10.1021/bi701322g. Epub 2008 Jan 19. Erratum in:Biochemistry. 2008 Oct 7;47(40):10827. PubMed PMID: 18205394.

11: Wehofsky N, Pech A, Liebscher S, Schmidt S, Komeda H, Asano Y, Bordusa F.D-amino acid specific proteases and native all-L-proteins: a convenientcombination for semisynthesis. Angew Chem Int Ed Engl. 2008;47(29):5456-60. doi: 10.1002/anie.200800340. PubMed PMID: 18551688.

12:  Günther R, Elsner C, Schmidt S, Hofmann HJ, Bordusa F. On the rational  designof substrate mimetics: The function of docking approaches for the  prediction ofprotease specificities. Org Biomol Chem. 2004 May  21;2(10):1442-6. Epub 2004 Apr 16. PubMed PMID: 15136799.

13: Machova Z, von Eggelkraut-Gottanka R, Wehofsky N, Bordusa F, Beck-SickingerAG.  Expressed enzymatic ligation for the semisynthesis of chemically  modifiedproteins. Angew Chem Int Ed Engl. 2003 Oct 20;42(40):4916-8. PubMed PMID:14579438.

14:  Wehofsky N, Koglin N, Thust S, Bordusa F. Reverse proteolysis promoted  by in situ generated peptide ester fragments. J Am Chem Soc. 2003  May21;125(20):6126-33. PubMed PMID: 12785843.

15: Wehofsky N, Thust S, Burmeister J, Klussmann S, Bordusa F.all-D-Polypeptides: novel targets for semisynthesis. Angew Chem Int Ed Engl. 2003Feb 10;42(6):677-9. PubMed PMID: 12575004.

16: Rall K, Bordusa F. Substrate mimetics-specific peptide ligases: studies onthe synthetic utility of a zymogen and zymogen-like enzymes. J Org Chem. 2002 Dec13;67(25):9103-6. PubMed PMID: 12467440.

17: Bordusa F. Proteases in organic synthesis. Chem Rev. 2002Dec;102(12):4817-68. Review. PubMed PMID: 12475208.

18: Wehofsky N, Löser R, Buchynskyy A, Welzel P, Bordusa F. Synthesis ofneo-peptidoglycans: an unexpected activity of proteases. Angew Chem Int Ed Engl. 2002 Aug 2;41(15):2735-8. PubMed PMID: 12203469.

19:  Bordusa F. Substrate mimetics in protease catalysis:  characteristics,kinetics, and synthetic utility. Curr Protein Pept Sci.  2002 Apr;3(2):159-80.Review. PubMed PMID: 12188901.

20: Wehofsky N, Alisch M, Bordusa F. Synthesising protease-stable  isopeptides by proteases: an efficient biocatalytic approach on the  basis of a new type ofsubstrate mimetics. Chem Commun (Camb). 2001 Sep  7;(17):1602-3. PubMed PMID:12240402.

21: Bordusa F. Enzymes for peptide cyclization. Chembiochem. 2001 Jun1;2(6):405-9. PubMed PMID: 11828470.

22:  Xu S, Rall K, Bordusa F. Enzymatic coupling of specific peptides  atnonspecific ligation sites: effect of Asp189Glu mutation in trypsin on  substrate mimetic-mediated reactions. J Org Chem. 2001 Mar 9;66(5):1627-32. PubMed PMID:11262106.

23: Kockskämper J, Sendhoff K, Erlenkamp S, Bordusa F, Cerovsky V, Glitsch HG.Differences in the protein-kinase-A-dependent regulation of CFTR Cl- channels andNa+-K+ pumps in guinea-pig ventricular myocytes. Pflugers Arch. 2001Mar;441(6):807-15. PubMed PMID: 11316265.

24:  Grünberg R, Domgall I, Günther R, Rall K, Hofmann HJ, Bordusa F.  Peptide bondformation mediated by substrate mimetics. Structure-guidedoptimization of trypsinfor synthesis. Eur J Biochem. 2000 Dec;267(24):7024-30. PubMed PMID: 11106412.

25:  Müller N, Bordusa F. Assay of diverse protease activities on the basis  of asmall synthetic substrate. Anal Biochem. 2000 Nov 1;286(1):86-90. PubMed PMID:11038278.

26: Cerovský V, Kockskämper J, Glitsch HG, Bordusa F. Semisynthesis ofHt31(493-515): involvement of PKA-anchoring proteins in the regulation of thecAMP-dependent chloride current in heart cells. Chembiochem. 2000 Aug18;1(2):126-9. PubMed PMID: 11828407.

27:  Wehofsky N, Kirbach SW, Haensler M, Wissmann JD, Bordusa F.  Substratemimetics and freezing strategy: a useful combination that  broadens the scope ofproteases for synthesis. Org Lett. 2000 Jul  13;2(14):2027-30. PubMed PMID:10891221.

28: Wehofsky N, Wissmann J, Alisch M, Bordusa F. Engineering of substratemimetics as novel-type substrates for glutamic acid-specific endopeptidases:design, synthesis, and application. Biochim Biophys Acta. 2000 Jun15;1479(1-2):114-22. PubMed PMID: 11004534.

29: Günther R, Thust S, Hofmann HJ, Bordusa F. Trypsin-specificacyl-4-guanidinophenyl esters for alpha-chymotrypsin-catalysed reactionscomputational predictions, hydrolyses, and peptide bond formation. Eur J Biochem.2000 Jun;267(12):3496-501. PubMed PMID: 10848965.

30:  Bordusa F. Nonconventional amide bond formation catalysis: programming  enzymespecificity with substrate mimetics. Braz J Med Biol Res. 2000  May;33(5):469-85. Review. PubMed PMID: 10775878.

31: Cerovský V, Bordusa F. Protease-catalyzed fragment condensation via substratemimetic strategy: a useful combination of solid-phase peptide synthesis withenzymatic methods. J Pept Res. 2000 Apr;55(4):325-9. PubMed PMID: 10798378.

32:  Günther R, Stein A, Bordusa F. Investigations on the enzyme specificity  ofclostripain: a new efficient biocatalyst for the synthesis of peptide  isosteres. J Org Chem. 2000 Mar 24;65(6):1672-9. PubMed PMID: 10750491.

33:  Günther R, Bordusa F. Protease catalysis mediated by a substrate  mimetic: anovel enzymatic approach to the synthesis of carboxylic acid  amides. Chemistry.2000 Feb 4;6(3):463-7. PubMed PMID: 10747412.

34: Thormann M, Thust S, Hofmann HJ, Bordusa F. Protease-catalyzed  hydrolysis of substrate mimetics (inverse substrates): A new approach  reveals a new mechanism. Biochemistry. 1999 May 11;38(19):6056-62. PubMed PMID: 10320331.

35: Wehofsky N, Bordusa F. Programming of enzyme specificity by substratemimetics: investigations on the Glu-specific V8 protease reveals a novel general principle of biocatalysis. FEBS Lett. 1999 Jan 25;443(2):220-4. PubMed PMID:9989609.

36:  Bordusa F, Jakubke HD. The specificity of prolyl endopeptidase  fromFlavobacterium meningoseptum: mapping the S' subsites by positional  scanning via acyl transfer. Bioorg Med Chem. 1998 Oct;6(10):1775-80. PubMed PMID: 9839007.

37:  Bordusa F, Ullmann D, Jakubke HD. Subsite specificity studies on the  unusual cysteine protease clostripain: charged residues in the P3  position indicate anarrow subsite region. Biol Chem. 1997  Oct;378(10):1193-8. PubMed PMID: 9372191.

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