Publications Dr. Johanna Mansfeld
Lux G, Mansfeld J, Ulbrich-Hofmann R (2014) Phospholipase A2-catalyzed acylation of lysophospholipids analyzed by experimental design. Enz Micr Technol (in press)
Mansfeld J, Schöpfel M, Lorenz J, Trutschel T, Heilmann I, Brandt W, Ulbrich-Hofmann R (2014) Probing selected structural regions in the plant secreted phospholipase A2 from Arabidopsis thaliana for their impact on stability and activity. Biochimie 101, 60-66
Oblozinsky, M., Bezakova, L., Mansfeld, J., Heilmann, I., and Ulbrich-Hofmann, R. (2013) "Differences in the effect of phosphatidylinositol 4,5-bisphosphate on the hydrolytic and transphosphatidylation activities of membrane-bound phospholipase D from poppy seedlings" Plant Physiol. Biochem. 69, 39-42
Mansfeld, J. (2013) "Stearolysin" in Handbook of Proteolytic Enzymes 3rd ed., Rawlings, N. D.and Salvesen, G. S. (eds), Elsevier
Mansfeld, J., Brandt, W., Haftendorn, R., Schöps, R., and Ulbrich-Hofmann, R. (2011) "Discrimination between the regioisomeric 1,2- and 1,3-diacylglycerophosphocholines by phospholipases." Chem. Phys. Lipids 164, 196-204
Oblozinski, M., Bezakova, L., Mansfeld, J., and Ulbrich-Hofmann, R. (2011) "The transphosphatidylation potential of a membrane-bound phospholipase D from poppy seedlings." Phytochem. 72, 160-165
Welker, S., Markert, Y., Köditz, J., Mansfeld, J., and Ulbrich-Hofmann, R. (2011) "Disulfide bonds of phospholipase A2 from bee venom yield discrete contributions to its conformational stability." Biochimie 93, 195-201
Dürrschmidt, P., Mansfeld, J., and Ulbrich-Hofmann, R. (2010) "Refolding of the non-specific neutral protease from Bacillus stearothermophilus proceeds via an autoproteolytically sensitive intermediate." Biophys. Chem. 147, 66-73.
Mansfeld, J. (2009) "Plant phospholipases A2: Perspectives on biotechnological applications." Biotechnol Lett. 31, 1373-1380
Mansfeld, J. and Ulbrich-Hofmann, R. (2009) "The modulation of phospholipase D activity in vitro." Biochim. Biophys. Acta 1791, 913 - 926.
Mansfeld, J. and Ulbrich-Hofmann, R. (2007) "Secretory phospholipase A2-alpha from Arabidopsis thaliana: functional parameters and substrate preferences." Chem. Phys. Lipids 150, 156-166.
Tripathi, P., Lo Leggio, L., Mansfeld, J., Ulbrich-Hofmann, R., and Kayastha, A. M. (2007) "α-Amylase from mung beans (Vigna radiata) - Correlation of biochemical properties and tertiary structure by homology modelling." Phytochemistry 68, 1623-1631
Markert, Y., Mansfeld, J., Schierhorn, A., Rücknagel, K. P., and Ulbrich-Hofmann, R. (2007) "Production of synthetically created phospholipase A2 variants with industrial impact." Biotechnol. Bioeng. 98, 48-59.
Mansfeld, J. and Ulbrich-Hofmann, R. (2007) "The stability of engineered thermostable neutral proteases from Bacillus stearothermophilus in organic solvents and detergents." Biotechnol. Bioeng. 97, 672-679.
Lerchner, A., Mansfeld, J., Kuppe, K., and Ulbrich-Hofmann, R. (2006) "Probing conserved amino acids in phospholipase D (Brassica oleracea var. capitata) for their importance in hydrolysis and transphosphatidylation activity." Protein Eng. Des. Sel. 19, 443-452.
Mansfeld, J., Gebauer, S., Dathe, K., and Ulbrich-Hofmann, R. (2006) "Secretory phospholipase A2 from Arabidopsis thaliana: Insights into the three-dimensional structure and the amino acids involved in catalysis." Biochemistry 45, 5687-5694.
Lerchner, A., Mansfeld, J., Schäffner, I., Schoeps, R., Beer, H. K., and Ulbrich-Hofmann, R. (2005) "Two highly homologous phospholipase D isoenzymes from Papaver somniferum L. with different transphosphatidylation potential" Biochim. Biophys. Acta 1737, 94-101.
Dürrschmidt, P., Mansfeld, J., and Ulbrich-Hofmann, R. (2005) "An engineered disulfide bridge mimics the effect of calcium to protect neutral protease against local unfolding", FEBS J. 272, 1523-1534.
Mansfeld, J., Petermann, E., Dürrschmidt, P., and Ulbrich-Hofmann, R. (2005) "The propeptide is not required to produce catalytically active neutral protease from Bacillus stearothermophilus", Protein Expr. Purif. 39, 219-228.
Schäffner, I., Rücknagel, K.-P., Mansfeld, J., and Ulbrich-Hofmann, R. (2002) "Genomic structure, cloning and expression of two phospholipase D isoenzymes from white cabbage", Eur. J. Lipid Sci. Technol. 104, 79-87.
Schöps, R., Schierhorn, A., Schäffner, I., Mansfeld, J., and Ulbrich-Hofmann, R. (2002) "Identification of phospholipase D from cabbage as N-terminally acetylated PLD2", J. Protein Chem. 21, 407-411.
Ulbrich-Hofmann, R., Mansfeld, J., Arnold, U., Köditz, J., Dürrschmidt, P., Markert, Y. (2002) "Lokal contra global - Strukturloops als Determinanten der Proteinstabilität", BIOforum 25, 14-17.
Kühn, D., Dürrschmidt, P., Mansfeld, J., and Ulbrich-Hofmann, R. (2002) "Boilysin and thermolysin in dipeptide synthesis: a comparative study", Biotechnol. Appl. Biochem. 36, 71-76.
Markert, Y., Köditz, J., Mansfeld, J., Arnold, U., and Ulbrich-Hofmann, R. (2001) "Increased proteolytic resistance of ribonuclease A by protein engineering", Protein Eng. 14, 791-796.
Dürrschmidt, P., Mansfeld, J., and Ulbrich-Hofmann, R. (2001) "Differentiation between conformational and autoproteolytic stability of the neutral protease from Bacillus earothermophilus containing an engineered disulfide bond ", Eur. J. Biochem. 268, 3612-3618.
Mansfeld, J., and Ulbrich-Hofmann, R. (2000) "Site-specific and random immobilization of thermolysin-like proteases reflected in the thermal inactivation kinetics", Biotechnol. Appl. Biochem. 32, 189-195.
Ulbrich-Hofmann, R., Arnold, U., and Mansfeld, J. (1999) "The concept of the unfolding region for approaching the mechanisms of enzyme stabilization", J. Mol. Cat. B7, 125-131.
Van den Burg, B., de Kreij, A., Van der Veek, P., Mansfeld, J., and Venema, G. (1999) "Characterization of a novel stable biocatalyst obtained by protein engineering", Biotechnol. Appl. Biochem.30, 35-40.
Mansfeld, J., Vriend, G., Van den Burg, B., Eijsink, V. G. H., and Ulbrich-Hofmann, R. (1999) "Probing the unfolding region in a thermolysin-like protease by site-specific immobilization", Biochemistry 38, 8240-8245.
Mansfeld, J., Vriend, G., Van den Burg, B., Venema, G.,Eijsink, V.G.H.,Ulbrich-Hofmann, R. (1998) "The effect of site-specific immobilization on the thermal stability of thermolysin-like neutral proteases", Progr. Biotechnol. 15, 513-516.
Mansfeld, J., and Dautzenberg, H. (1997) "Coimmobilization of enzymes and cells in polyelectrolyte complex capsules", In: Methods in Biotechnol., Immobilization of Enzymes and Cells (G. F. Bickerstaff, ed.), Humana Press, New Jersey pp. 319-326.
Mansfeld, J., and Dautzenberg, H. (1997) "Immobilization of cells in polyelectrolyte complex capsules", In: Methods in Biotechnol., Immobilization of Enzymes and Cells (G. F. Bickerstaff, ed.), Humana Press, New Jersey pp. 309-318.
Mansfeld, J., Vriend, G., Dijkstra, B. W., Veltman, O. R., Van den Burg, B., Venema, G., Ulbrich-Hofmann R., and Eijsink, V. G. H. (1997) "Extreme Stabilization of a thermolysin-like protease by an engineered disulfide bond", J. Biol. Chem.
272, 11152-11156.
Veltman, O. R., Vriend, G., Hardy, F., Mansfeld, J., Van den Burg, B., Venema, G., and Eijsink, V. G. H. (1997) "Mutational analysis of a surface area that is critical for the thermal stability of thermolysin-like proteases", Eur. J. Biochem. 248, 433-440.