Publikationen der AG Wahle
2023
Pekovic, F., Rammelt, C., Kubíková, J., Metz, J., Jeske, M., Wahle E. (2023)RNA binding proteins Smaug and Cup induce CCR4-NOT-dependent deadenylation of the nanos mRNA in a reconstituted system. Nucleic Acids Res. Online ahead of print
2022
Schmidt, M., Kluge, F., Sandmeir, F., Kühn, U., Schäfer, P., Tüting, C., Ihling, C., Conti, E., Wahle, E. (2022) Reconstitution of 3' end processing of mammalian pre-mRNA reveals a central role of RBBP6. Genes Dev. 36, 195-209. doi:10.1101/gad.349276.121
2020
Kluge, F., Götze, M., Wahle, E. (2020) Establishment of 5’-3’ interactions in mRNA independent of a continuous ribose-phosphate backbone. RNA 26, 613-628.
Cao, W. X., Kabelitz, S. Gupta, M., Yeung, E., Lin, S., Rammelt, C ., Ihling, C., Pekovic, F., Low, T. C. H., Siddiqui, N. U., Cheng, M. H. K., Anger, S., Smibert, C. A., Wühr, M., Wahle, E.*, Lipshitz, H. D.* (2020) Precise temporal regulation of post-transcriptional repressors is required for an orderly Drosophila maternal-to-zygotic transition. Cell Rep. 31, 107783. *corresponding authors
2018
Schäfer, P., Tüting, C., Schönemann, L., Kühn, U., Treiber, T., Treiber, N., Ihling, C., Graber, A., Keller, W., Meister, G., Sinz, A., Wahle, E. (2018) Reconstitution of mammalian cleavage factor II involved in 3' processing of mRNA precursors. RNA 24, 1721-1737.
2017
Kühn, U., Buschmann, J., Wahle, E. (2017) The nuclear poly(A) binding protein of mammals, but not of fission yeast, participates in mRNA polyadenylation. RNA 23, 473-482.
Gehring, N. H., Wahle, E., Fischer, U. (2017) Deciphering the mRNP code: RNA-bound determinants of post-transcriptional gene regulation. Trends Biochem. Sci. 42, 369-382.
Götze, M., Dufourt, J., Ihling, C., Rammelt, C., Pierson, S., Sambrani, N., Temme, C., Sinz, A., Simonelig, M., Wahle, E. (2017) Translational repression of the Drosophila nanos mRNA involves the RNA helicase Belle and RNA coating by Me31B and Trailer hitch. RNA 23, 1552-1568.
2016
Harnisch, C., Cuzic-Feltens, S., Dohm, J. C., Götze, M., Himmelbauer, H., Wahle, E. (2016) Oligoadenylation of 3' decay intermediates promotes cytoplasmic mRNA degradation in Drosophila cells. RNA 22, 428-442.
2015
Götze, M., Pettelkau, J., Fritzsche, R., Ihling, C.H., Schäfer, M., Sinz, A.(2015) Automated Assignment of MS/MS Cleavable Cross-Links in Protein 3D-Structure Analysis. J Am Soc Mass Spectrom. 26, 83-97.
Fiedler, M., Rossmanith, W., Wahle, E., Rammelt, C. (2015) Mitochondrial poly(A) polymerase is involved in tRNA repair. Nucleic Acids Res. 43, 9937-9949.
2014
Moritz, B., Wahle, E. (2014) Simple methods for the 3' biotinylation of RNA. RNA 20, 421-427.
Götze, M., Wahle, E. (2014) Smaug destroys a huge treasure. Genome biology 15: 101.
Temme, C., Wahle, E. (2014) Assaying mRNA deadenylation in vivo. Methods Mol Biol. 1125, 313-324.
Jeske, M., Temme, C., Wahle, E. (2014) Assaying mRNA deadenylation in vitro. Methods Mol Biol. 1125, 297-311.
Monecke, T., Buschmann, J., Neumann, P., Wahle, E., Ficner, R. (2014) Crystal structures of the novel cytosolic 5'-nucleotidase IIIB explain its preference for m7GMP. PLoS One. 9:e90915. doi: 10.1371
Temme, C., Simonelig, M., Wahle, E. (2014) Deadenylation of mRNA by the CCR4-NOT complexin Drosophila: molecular and developmental aspects. Front Genet. 5, 143.
Stubbs, M.T., Wahle, E. (2014) Deadenylation-a piece of PANcake. EMBO J. 33, 1503-1505.
Moritz, B., Lilie, H., Naarmann-de Vries, I.S., Urlaub, H., Wahle, E., Ostareck-Lederer, A., Ostareck, D.H. (2014) Biophysical and biochemical analysis of hnRNP K: arginine methylation, reversible aggregation and combinatorial binding to nucleic acids. Biol Chem. 395, 837-853.
Schönemann, L., Kühn, U., Martin, G., Schäfer, P., Gruber A. R., Keller,
W., Zavolan, M., Wahle, E. (2014) Reconstitution of CPSF active in
polyadenylation: Recognition of the polyadenylation signal by WDR33.
Genes & Development 28, 2381-2393
2013
Harnisch, C., Moritz, B., Rammelt, C., Temme, C., Wahle, E. (2013) Activity and function of deadenylases. In: G. Chanfreau, F. Tamanoi, eds.: The Enzymes, Vol. 31, Eukaryotic RNases and their partners in RNA degradation and biogenesis, Part A. Burlington: Academic Press, pp. 181-211.
Buschmann, J., Moritz, B., Jeske, M., Lilie, H., Schierhorn, A., Wahle, E. (2013) Identification of Drosophila and human 7-methyl GMP-specific nucleotidases. J. Biol. Chem. 288, 2441-2451
Wahle E, Winkler GS. (2013) RNA decay machines: deadenylation by the Ccr4-not and Pan2-Pan3 complexes.Biochim Biophys Acta. 1829, 561-570.
2012
Berndt, H., Harnisch, C., Rammelt, C., Stöhr, N., Zirkel, A., Dohm, J. C., Himmelbauer, H., Tavanez, J.-P., Hüttelmaier, S., Wahle, E. (2012) Maturation of mammalian H/ACA box snoRNAs: PAPD5-dependent adenylation and PARN-dependent trimming. RNA, 18, 958-972.
Jenal, M., Elkon, R., Loayza-Puch, F., van Haaften, G., Kühn, U., Menzies, F.M., Vrielink, J.A., Bos, A.J., Drost, J., Rooijers, K., Rubinsztein, D.C., Agami, R. (2012) The poly(a)-binding protein nuclear 1 suppresses alternative cleavage and polyadenylation sites. Cell 149, 538-53.
Winter, R., Kühn, U., Hause, G., Schwarz, E. (2012) Poly-Alanine-independent conformational conversion of the nuclear poly(A) binding protein 1 (PABPN1). J. Biol. Chem. 287: 22662-71.
Kölbel, K., Ihling, C., Kühn, U., Neundorf, I., Otto, S., Stichel, J., Robaa, D., Beck-Sickinger, A.G., Sinz, A., Wahle, E. (2012) Peptide backbone conformation affects the substrate preference of protein arginine methyltransferase I. Biochemistry 51: 5463-75.
2011
Jeske, M., Moritz B., Anders, A., Wahle, E. (2011) Smaug assembles an ATP-dependent stable complex repressing nanos mRNA translation at multiple levels. EMBO J. 30, 90–103.
Fronz, K., Güttinger, S., Burkert, K., Kühn, U., Stöhr, N., Schierhorn, A., Wahle, E (2011) Arginine methylation of the nuclear poly(a) binding protein weakens the interaction with its nuclear import receptor, transportin. J. Biol. Chem. 286, 32986-32994.
Eckmann, C. R., Rammelt, C., Wahle, E. (2011) Control of poly(A) tail length. WIREs RNA 2011, 2: 348-361.
Götze, M., Pettelkau, J., Schaks, S., Bosse, K., Ihling, C.H.,Krauth, F., Fritzsche, R., Kühn, U., Sinz, A. (2011) StavroX—A Software for Analyzing Crosslinked Products in Protein Interaction Studies. J. Am. Soc. Mass Spectrom. 23, 76-87.
2010
Temme, C., Zhang, L., Kremmer, E., Ihling, C., Chartier, A., Sinz, A., Simonelig, M., Wahle, E. (2010) Subunits of the Drosophila CCR4-NOT complex and their roles in mRNA deadenylation. RNA 16, 1356-1370
2009
Hägele, S., Kühn, U., Böning, M., Katschinski, D. M. (2009) Cytoplasmic polyadenylation element binding protein (CPEB)1 and 2 bind to the HIF-1α mRNA 3`UTR and modulate HIF-1α protein expression. Biochemical Journal 417, 235-246.
Weidensdorfer, D., Stöhr, N., Baude, A., Lederer, M., Köhn, M., Schierhorn, A., Buchmeier, S., Wahle, E., Hüttelmaier, S. (2009) Control of c-myc mRNA stability by IGF2BP1 associated cytoplasmic RNPs. RNA 15, 104-115.
Temme, C., Weissbach, R., Lilie, H., Wilson, C., Meinhart, A., Meyer, S., Golbik, R., Schierhorn, A., Wahle, E. (2009) The Drosophila melanogaster gene CG4930 encodes a high affinity inhibitor for endonuclease G. J. Biol. Chem., 284, 8337-8348.
Kölbel, K., Ihling, C., Bellmann-Sickert, K., Neundorf, I., Beck-Sickinger, A. G., Sinz, A., Kühn, U., Wahle, E. (2009) Type I arginine methyl transferases PRMT1 and 3 act distributively. J. Biol. Chem., 284, 8274-8282
Kühn, U., Gündel, M. Knoth, A. Kerwitz; Y. Rüdel, S. Wahle, E. (2009) Poly(A) tail length is controlled by the nuclear poly(A) binding protein regulating the interaction between poly(A) polymerase and the cleavage and polyadenylation specificity factor. J. Biol. Chem., 284, 22803-22814.
Loll, B., Gebhardt, M., Wahle, E., Meinhart, A. (2009) Crystal structure of the EndoG/EndoGI complex: Mechanism of EndoG inhibition. Nucleic Acids Res., 3/, 7312-7320.
2008
Lodderstedt, G., Sachs, R., Faust, J., Bordusa, F., Kühn, U., Golbik, R., Kerth, A., Wahle, E., Balbach, J., Schwarz, E. (2008) Hofmeister Salts and Potential Therapeutic Compounds Accelerate in Vitro Fibril Formation of the N-Terminal Domain of PABPN1 Containing a Disease-Causing Alanine Extension. Biochemistry 47, 2181-2189.
Fronz, K., Otto,S., Kölbel, K., Kühn, U., Friedrich, H., Schierhorn, A., Beck-Sickinger, A.-G., Ostareck-Lederer, A., Wahle, E. (2008) Promiscuous modification of the nuclear poly(A) binding protein by multiple protein arginine methyl transferases does not affect the aggregation behavior. J. Biol. Chem. 283, 20408-20420
Jeske, M., Wahle, E. (2008) Cell-free deadenylation assays using Drosophila embryo extracts. Methods Enzymol. 448, 107-118.
Meyer, S., Urbanke, C., Wahle, E. (2002) Equilibrium studies on the association of the nuclear poly(A) binding protein with poly(A) of different lengths. Biochem. 41, 6082-6089.
2007
Wahle, E. (2007) Wrong PH for RNA degradation. Nature Structural & Molecular Biology 14, 5-7.
Adolph, D., Flach, N., Mueller, K., Ostareck, D. H., Ostareck-Lederer, A. (2007) Deciphering the cross talk between hnRNP K and c-Src: the c-Src activation domain in hnRNP K is destinct from a second interaction site. Molecular and Cellular Biology 27, 1758-1770.
Bönisch, C., Temme, C., Moritz, B, Wahle, E. (2007) Degradation of hsp70 and other mRNAs in Drosophila via the 5`-3` pathway and its regulation by heat shock. J. Biol. Chem. 283, 21818-21828
2006
Ostareck-Lederer, A., Ostareck, D.H., Rücknagel, K.P., Schierhorn, A., Moritz, B., Hüttelmaier, S., Flach, N., Handoko, L. and Wahle, E. (2006) Asymmetric arginine dimethylation of hnRNP K by PRMT1 inhibits its interaction with c-Src. J. Biol. Chem. 281, 11115-11125.
Jeske, M., Meyer, S., Temme, C., Freudenreich, D., Wahle, E. (2006) Rapid ATP-dependent deadenylation of nanos mRNA in a cell-free system from Drosophila embryos. J. Biol. Chem. 281, 25124-25133.
Wahle, E., Gehring, N. H. (2006) Polyadenylation. In: Ruckpaul, K. und Ganten, D. (Herausg.), Encyclopedic Reference of Genomics and Proteomics in Molecular Medicine. Springer Verlag, Heidelberg.
Stöhr, N., Lederer, M., Reinke, C., Meyer, S., Hatzfeld, M., Singer, R. H., Hüttelmaier, S. (2006) ZBP1 regulates mRNA stability during cellular stress. J. Cell Biol., 175, 527-534.
Messias, A., Harnisch, C., Ostareck-Lederer, A., Sattler, M. and Ostareck, D.H. (2006) The DICE-binding activity of KH domain 3 of hnRNP K is affected by c-Src mediated tyrosine phosphorylation. J. Mol. Biol. 361, 470-481
2005
Benoit B, Mitou G, Chartier A, Temme C, Zaessinger S, Wahle E, Busseau I, Simonelig M. (2005) An essential cytoplasmic function for the nuclear poly(A) binding protein, PABP2, in poly(A) tail length control and early development in Drosophila. Dev Cell. 9, 511-22.
Wu M, Reuter M, Lilie H, Liu Y, Wahle E, Song H. (2005) Structural insight into poly(A) binding and catalytic mechanism of human PARN. EMBO J. 24, 4082-93.
Boehringer, D., Thermann, R., Ostareck-Lederer, A., Lewis, J.D. and Stark, H. (2005) Structure of the Hepatitis C virus IRES bound to the human 80S ribosome: Remodelling of the HCV IHRES. Structure 13, 1695-1706
2004
Kühn, U., Wahle, E. (2004) Structure and function of poly(A) binding proteins. Biochim. Biophys. Acta 1678, 67-84.
Temme, C., Zaessinger, S., Meyer, S., Simonelig, M., Wahle, E. (2004) A complex containing the CCR4 and CAF1 proteins is involved in mRNA deadenylation in Drosophila. EMBO J. 24, 4082-93.
Ostareck-Lederer, A. and Ostareck, D.H. (204) Control of mRNA translation and stability in haematopoietic cells: Functions of hnRNPs K and E1/2. Biol. Cell 96, 407-411 .
Ostareck-Lederer, A., Clauder-Münster, S., Thermann, R., Polycarpou-Schwarz, M., Gentzel, M., Wilm, M. and Lewis, J.D. (2004) The role of RNA interference in drug target validation: application to Hepatitis C. In: RNA Interference: From Basic Science to Drug Development. (ed. K. Appasani), Cambridge University Press, 318-330.
Ostareck-Lederer, A. and Ostareck, D.H. (2004) Cellular signals controlling post-transcriptional regulation of reticulocyte-15-lipoxygenase (r15-LOX) expression during erythroid cell maturation. Nova Acta Leopoldina 18, 163-178.
Thoma, C., Ostareck-Lederer, A. and Hentze, M.W. (2004) A poly(A) tail responsive in vitro translation system in HeLa cells. In: mRNA Processing and Metabolism – Methods and Protocols. (ed. D.R. Schoenberg), The Human Press Inc. New York 2004, 171-180
2003
Kühn, U., Nemeth, A., Meyer, S., Wahle, E. (2003) The RNA binding domains of the nuclear poly(A) binding protein. J. Biol. Chem. 278, 16916-16925.
Kerwitz, Y., Kühn, U., Lilie, H., Knoth, A., Scheuermann, T., Friedrich, H., Schwarz, E., Wahle, E. (2003) Stimulation of poly(A) polymerase through a direct interaction with the nuclear poly(A) binding protein allosterically regulated by poly(A). EMBO J. 22, 3705-3714.
Scheuermann, T., Schulz, B., Blume, A., Wahle, E., Rudolph, R., Schwarz, E. (2003) Trinucleotide expansions leading to an extended poly-L-alanine segment in the poly(A) binding protein PABPN1 cause fibril formation. Protein Science 12, 2685-2692.
Evans J.R., Mitchell, S.A., Spriggs, K.A., Ostrowsky, J., Bomsztyk, K., Ostareck, D.H., and Willis, A.E. (2003) Members of the poly rC binding protein family stimulate the activity of the c-myc internal ribosome entry segment in vitro and in vivo. Oncogene 22, 8012-8020
2002
Calapez, A., Pereira, H. M., Calado, A., Braga, J., Rino, J., Carvalho, C., Tavanez, J. P., Wahle, E., Rosa, A. C., Carmo- Fonseca, M. (2002) The intranuclear mobility of messenger RNA binding proteins is ATP-dependent and temperature- sensitive. J. Cell Biol. 159, 795-805.
Juge, F., Zaessinger, S., Temme, C., Wahle, E., Simonelig, M. (2002) Control of poly(A) polymerase level is essential to cytoplasmic polyadenylation and early development in Drosophila. EMBO J. 21, 6603-6613.
Meyer, S., Urbanke, C., Wahle, E. (2002) Equilibrium studies on the association of the nuclear poly(A) binding protein with poly(A) of different lengths. Biochemistry 41, 6082-6089.
van Dijk, E., Cougot, N., Meyer, S., Babajko, S., Wahle, E., Séraphin, B. (2002) The human Dcp2 protein: a catalytically active mRNA decapping enzyme localized in specific cytoplasmic structures. EMBO J. 21, 6915-6924.
2000
Dehlin, E., Wormington, M., Körner, C. G., Wahle, E. (2000) Cap-dependent deadenylation of mRNA. EMBO J., 19, 1079-1086
Calado, A., Kutay, U., Kühn, U., Wahle, E., Carmo-Fonseca, M. (2000) Deciphering the cellular pathway for transport of poly(A)-binding protein II. RNA, 6, 245-256
Keller, R. W., Kühn, U., Aragon, M., Bornikova, L., Wahle, E., Bear, D. G. (2000) The nuclear poly(A) binding protein, PABP2, forms an oligomeric particle covering the length of the poly(A) tail. J. Mol. Biol., 297, 569-583
Winstall, E., Sadowski, M., Kühn, U., Wahle, E., Sachs, A. B. (2000) The Saccharomyces cerevisiae RNA binding protein rbp29 functions in cytoplasmic mRNA metabolism. J. Biol. Chem. 275, 21817-21826
Calado, A., Tomè, F. M. S., Brais, B., Rouleau, G. A., Kühn, U., Wahle, E., Carmo-Fonseca, M. (2000) Nuclear inclusions in oculopharyngeal muscular dystrophy consist of poly(A) binding protein 2 aggregates which sequester poly(A) RNA. Hum. Mol. Genet. 9, 2321-2328.
1999
Smith, J. J., Rücknagel, P., Schierhorn, A., Nemeth, A., Tang, J., Linder, M., Herschman, H. R., Wahle, E. (1999) Unusual sites of arginine methylation in poly(A)-binding protein II and in vitro methylation by protein arginine methyltransferases PRMT1 and PRMT3. J. Biol. Chem. 274, 13229-13234
Wahle, E., Rüegsegger, U. (1999) 3` end processing of pre-mRNA in eucaryotes. FEMS Microbiology Rev. 23, 277-295
Benoit, B., Nemeth, A., Aulner, N., Kühn, U., Simonelig, M., Wahle, E., Bourbon, H.-M. (1999) The Drosophila poly(A) binding protein II is ubiquitous throughout Drosophila development and has the same function in mRNA polyadenylation as its bovine homolog in vitro. Nucleic Acids Res. 27, 3771-3778
Buiting, K., Körner, C., Ulrich, B., Wahle, E., Horsthemke, B. (1999) The human gene for the poly(A)-specific ribonuclease (PARN) maps to 16p13 and has a truncated copy in the Prader-Willi/Angelman syndrome region on 15q11-q13. Cytogen. Cell Genet. 87, 125-131
1998
Körner, C. G., Wormington, M., Muckenthaler, M., Schneider, S., Dehlin, E., Wahle, E. (1998) The deadenylating nuclease (DAN) is involved in poly(A) tail removal during the meiotic maturation of Xenopus Oocytes. EMBO J. 17, 5427-5437
1997
Wittmann, T., Wahle, E. (1997) Purification and characterization of full-length mammalian poly(A) polymerase. Biochim. Biophys. Acta 1350, 293-305
Wahle, E., Kühn, U. (1997) The mechanism of 3`-cleavage and polyadenylation of eucaryotic pre-mRNA. Progr. Nucleic Acid Res. Mol. Biol. 57, 41-71
Wahle, E., Keller, W. (1997) Capping, methylation, and 3`-end formation of messenger RNA precursors. In: A. Krainer (Herausgeber), Frontiers in Molecular Biology: mRNA Processing, S. 280-309. IRL Press, Oxford
Körner, C., Wahle, E. (1997) Poly(A) tail shortening by a mammalian poly(A)-specific 3`-exoribonuclease. J. Biol. Chem. 272, 10448-10456
1996
Wahle, E., Keller, W. (1996) The biochemistry of polyadenylation. Trends Biochem. Sci. 21, 247-250
1995
Wahle, E. (1995) Poly(A) tail length control is caused by termination of processive synthesis. J. Biol. Chem. 270, 2800-2808
Wahle, E. (1995) 3`-end cleavage and polyadenylation of mRNA precursors. Biochim. Biophys. Acta 1261, 183-194
Nemeth, A., Krause, S., Blank, D., Jenny, A., Jenö, P., Lustig, A., Wahle, E. (1995) Isolation of genomic and cDNA clones encoding bovine poly(A) binding protein II. Nucleic Acids Res. 23, 4034-4041